College of Environmental Sciences and Engineering, Peking University, Beijing, China.
J Zhejiang Univ Sci B. 2011 Sep;12(9):760-8. doi: 10.1631/jzus.B1000440.
Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in a municipal solid waste (MSW) composting site. Knowledge of changes in microbial structure is useful to identify particular PAH degraders. However, the microbial community in the MSW composting soil and its change associated with prolonged exposure to PAHs and subsequent biodegradation remain largely unknown. In this study, anthracene was selected as a model compound. The bacterial community structure was investigated using terminal restriction fragment length polymorphism (TRFLP) and 16S rRNA gene clone library analysis. The two bimolecular tools revealed a large shift of bacterial community structure after anthracene amendment and subsequent biodegradation. Genera Methylophilus, Mesorhizobium, and Terrimonas had potential links to anthracene biodegradation, suggesting a consortium playing an active role.
多环芳烃（PAHs）是城市固体废物（MSW）堆肥场中的常见污染物。了解微生物结构的变化有助于识别特定的 PAH 降解菌。然而，MSW 堆肥土壤中的微生物群落及其与长时间暴露于 PAHs 相关的变化以及随后的生物降解在很大程度上仍不清楚。在本研究中，蒽被选为模型化合物。使用末端限制性片段长度多态性（TRFLP）和 16S rRNA 基因克隆文库分析来研究细菌群落结构。这两种双分子工具揭示了蒽添加和随后的生物降解后细菌群落结构的巨大变化。甲基杆菌属、根瘤菌属和地杆菌属与蒽的生物降解具有潜在联系，表明一个联合体发挥了积极作用。
