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蒽添加对污染和未污染场地土壤微宇宙中微生物群落结构的影响。

Impact of anthracene addition on microbial community structure in soil microcosms from contaminated and uncontaminated sites.

机构信息

College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

出版信息

Biomed Environ Sci. 2011 Oct;24(5):543-9. doi: 10.3967/0895-3988.2011.05.014.

DOI:10.3967/0895-3988.2011.05.014
PMID:22108422
Abstract

OBJECTIVE

This paper aims to investigate the impact of anthracene addition on microbial community in agricultural soil irrigated with tap water or reclaimed wastewater.

METHODS

The changes of microbial community were characterized by terminal restriction fragment length polymorphism in combination with 16S rRNA gene clone library analysis.

RESULTS

A significant change in microbial community composition was observed during the biodegradation of anthracene, with dominantly enriched members from the genus Methylophilus.

CONCLUSION

This work might be useful for developing techniques for the isolation of novel putative PAH degrader.

摘要

目的

本研究旨在探讨蒽添加对以自来水或再生水灌溉的农业土壤微生物群落的影响。

方法

采用末端限制性片段长度多态性结合 16S rRNA 基因克隆文库分析的方法来表征微生物群落的变化。

结果

在蒽的生物降解过程中，微生物群落组成发生了显著变化，其中主要富集了甲基杆菌属的成员。

结论

这项工作可能有助于开发分离新型潜在多环芳烃降解菌的技术。

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