School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, Wales, UK.
Sci Total Environ. 2011 Oct 15;409(22):4781-7. doi: 10.1016/j.scitotenv.2011.07.041. Epub 2011 Sep 3.
This study investigated the effects of reactive oxygen species (ROS) generated as a function of the physicochemistry of incense particulate matter (IPM), diesel exhaust particles (DEP) and carbon black (CB). Microscopical and elemental analyses were used to determine particle morphology and inorganic compounds. ROS was determined using the reactive dye, Dichlorodihydrofluorescin (DCFH), and the Plasmid Scission Assay (PSA), which determine DNA damage. Two common types of soot were observed within IPM, including nano-soot and micro-soot, whereas DEP and CB mainly consisted of nano-soot. These PM were capable of causing oxidative stress in a dose-dependent manner, especially IPM and DEP. A dose of IPM (36.6-102.3μg/ml) was capable of causing 50% oxidative DNA damage. ROS formation was positively correlated to smaller nano-soot aggregates and bulk metallic compounds, particularly Cu. These observations have important implications for respiratory health given that inflammation has been recognised as an important factor in the development of lung injury/diseases by oxidative stress. This study supports the view that ROS formation by combustion-derived PM is related to PM physicochemistry, and also provides new data for IPM.
本研究调查了作为香颗粒物质（IPM）、柴油尾气颗粒（DEP）和炭黑（CB）物理化学特性函数产生的活性氧物质（ROS）的影响。使用显微镜和元素分析来确定颗粒形态和无机化合物。使用活性染料二氯二氢荧光素（DCFH）和质粒断裂测定（PSA）来测定 ROS，这两种方法可测定 DNA 损伤。在 IPM 中观察到两种常见类型的烟尘，包括纳米烟尘和微烟尘，而 DEP 和 CB 主要由纳米烟尘组成。这些 PM 能够以剂量依赖的方式引起氧化应激，尤其是 IPM 和 DEP。剂量为 36.6-102.3μg/ml 的 IPM 能够造成 50%的氧化 DNA 损伤。ROS 的形成与较小的纳米烟尘聚集体和块状金属化合物，特别是 Cu 呈正相关。这些观察结果对于呼吸健康具有重要意义，因为炎症已被认为是氧化应激导致肺损伤/疾病的一个重要因素。本研究支持了这样一种观点，即燃烧衍生的 PM 产生的 ROS 与 PM 的物理化学性质有关，并且为 IPM 提供了新的数据。
