van de Moer A, Salhi S L, Cherniak R, Pau B, Garrigues M L, Bastide J M
Unité de Recherche en Immunologie, Faculté de Pharmacie, Montpellier, France.
Res Immunol. 1990 Jan;141(1):33-42. doi: 10.1016/0923-2494(90)90099-k.
Six monoclonal antibodies (mAb) were produced by immunizing mice with a Cryptococcus neoformans serotype A spheroplast lysate (CNSL). Two mAb were of the IgG1 isotype; the others were IgM. The results obtained with one IgM mAb (CN6) are reported herein. This mAb recognized the four serotypes of C. neoformans and no cross-reactions were observed with extracts from Cryptococcus melibiosum, Candida albicans, Saccharomyces cerevisiae, Torulopsis glabrata or Trichosporon beigelii. Fractionation of the CNSL by gel filtration revealed that mAb CN6 recognized high molecular weight substances as well as a range of smaller molecules. Indirect ELISA inhibition studies showed that this mAb recognized substances in a cryptococcal culture filtrate. Inhibition studies and agglutination tests using latex beads sensitized with purified CN6 showed that CN6 strongly reacted with the C. neoformans serotype A cell envelope galactoxylomannan-mannoprotein complex (GAlXM-MP) and only weakly with the glucuronoxylomannan (GXM) component. These tests also showed that purified galactoxylomannan (GalXM) from C. neoformans serotype A was more reactive than purified mannoprotein (MP). An anti-GalXM mAb might be a useful tool for monitoring the clinical course of cryptococcal infections.
用新型隐球菌A血清型原生质体裂解物（CNSL）免疫小鼠，产生了六种单克隆抗体（mAb）。其中两种mAb为IgG1同种型；其他的为IgM。本文报道了一种IgM mAb（CN6）的实验结果。该mAb可识别新型隐球菌的四种血清型，且未观察到与蜜二糖隐球菌、白色念珠菌、酿酒酵母、光滑球拟酵母或白吉利丝孢酵母提取物发生交叉反应。通过凝胶过滤对CNSL进行分级分离显示，mAb CN6可识别高分子量物质以及一系列较小的分子。间接ELISA抑制研究表明，该mAb可识别隐球菌培养滤液中的物质。使用用纯化的CN6致敏的乳胶珠进行抑制研究和凝集试验表明，CN6与新型隐球菌A血清型细胞包膜半乳甘露聚糖 - 甘露蛋白复合物（GAlXM - MP）强烈反应，而与葡糖醛酸木聚糖甘露聚糖（GXM）成分反应较弱。这些试验还表明，来自新型隐球菌A血清型的纯化半乳甘露聚糖（GalXM）比纯化的甘露蛋白（MP）反应性更强。抗GalXM mAb可能是监测隐球菌感染临床病程的有用工具。
