Department of Physical Medicine and Rehabilitation, Christian Medical College, Vellore, Tamil Nadu, India.
Neurol India. 2011 Jul-Aug;59(4):566-72. doi: 10.4103/0028-3886.84339.
Olfactory ensheathing cells (OEC) are considered to be the most suitable cells for transplantation therapy in the central nervous system (CNS) because of their unique ability to help axonal regrowth and remyelination in the CNS. However, there are conflicting reports about the success rates with OEC.
This study was undertaken to evaluate the therapeutic effect of OEC in rat models using different cell dosages.
OECs harvested from the olfactory mucosa of adult white Albino rats were cultured. Spinal cord injury (SCI) was inflicted at the lower thoracic segment in a control and test group of rats. Two weeks later, OECs were delivered in and around the injured spinal cord segment of the test group of the rats. The outcome in terms of locomotor recovery of limb muscles was assessed on a standard rating scale and by recording the motor-evoked potentials from the muscles during transcranial electrical stimulation. Finally, the animals were sacrificed to assess the structural repair by light microscopy.
Wilcoxon signed rank test and Mann-Whitney U-test were used to compare the data in the control and the test group of animals. A P value of <0.05 was considered significant.
The study showed a moderate but significant recovery of the injured rats after OEC transplantation (P=0.005).
Transplantation of OECs along with olfactory nerve fibroblasts improved the motor recovery in rat models with SCI.
嗅鞘细胞（OEC）因其独特的帮助中枢神经系统（CNS）轴突再生和髓鞘形成的能力，被认为是中枢神经系统移植治疗中最适合的细胞。然而，关于 OEC 的成功率有相互矛盾的报道。
本研究旨在评估不同细胞剂量的 OEC 在大鼠模型中的治疗效果。
从成年白化大鼠嗅黏膜中采集 OEC 进行培养。在对照组和实验组大鼠的下胸段造成脊髓损伤（SCI）。2 周后，将 OEC 输送到实验组大鼠损伤的脊髓段内及周围。通过标准评分量表评估肢体肌肉运动恢复情况，并通过经颅电刺激记录肌肉运动诱发电位。最后，对动物进行安乐死，通过光镜评估结构修复情况。
采用 Wilcoxon 符号秩检验和 Mann-Whitney U 检验比较对照组和实验组动物的数据。P 值<0.05 被认为具有统计学意义。
研究表明，OEC 移植后大鼠的损伤有一定程度但显著的恢复（P=0.005）。
嗅鞘细胞与嗅神经成纤维细胞联合移植可改善 SCI 大鼠的运动恢复。
