Wang Dong, Zhang Jianjun
Department of Neurosurgery, Tianjin Fourth Central Hospital, Tianjin 300140, China.
Neural Regen Res. 2012 Apr 5;7(10):749-55. doi: 10.3969/j.issn.1673-5374.2012.10.005.
Following successful establishment of a rat model of spinal cord hemisection injury by resecting right spinal cord tissues, bone marrow stem cells were transplanted into the spinal cord lesions via the caudal vein while maintaining rectal temperature at 34 ± 0.5°C for 6 hours (mild hypothermia). Hematoxylin-eosin staining showed that astrocytes gathered around the injury site and formed scars at 4 weeks post-transplantation. Compared with rats transplanted with bone marrow stem cells under normal temperature, rats transplanted with bone marrow stem cells under hypothermia showed increased numbers of proliferating cells (bromodeoxyuridine-positive cells), better recovery of somatosensory-evoked and motor-evoked potentials, greater Basso, Beattie, and Bresnahan locomotor rating scores, and an increased degree of angle in the incline plate test. These findings suggested that hypothermia combined with bone marrow mesenchymal stem cells transplantation effectively promoted electrical conduction and nerve functional repair in a rat model of spinal cord hemisection injury.
通过切除右侧脊髓组织成功建立大鼠脊髓半切损伤模型后，将骨髓干细胞经尾静脉移植到脊髓损伤部位，同时将直肠温度维持在34±0.5°C达6小时（轻度低温）。苏木精-伊红染色显示，移植后4周星形胶质细胞聚集在损伤部位周围并形成瘢痕。与常温下移植骨髓干细胞的大鼠相比，低温下移植骨髓干细胞的大鼠增殖细胞（溴脱氧尿苷阳性细胞）数量增加，体感诱发电位和运动诱发电位恢复更好，Basso、Beattie和Bresnahan运动评分更高，倾斜板试验中的角度增加程度更大。这些发现表明，低温联合骨髓间充质干细胞移植可有效促进大鼠脊髓半切损伤模型中的电传导和神经功能修复。
