Institut für Analytische Chemie, Universität Leipzig, Linnéstr 3, 04103 Leipzig, Germany.
Anal Bioanal Chem. 2011 Nov;401(8):2651-6. doi: 10.1007/s00216-011-5351-2. Epub 2011 Sep 3.
We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 μm in height were generated. The microfluidic chips feature ion-permeable segregation walls between the electrode channels and the separation bed and hydrophilic surfaces. The performance of the chip is demonstrated by free-flow electrophoretic separation of fluorescent xanthene dyes and fluorescently labeled amino acids.
我们提出了一种快速且多功能的方法来制作功能微流控自由电泳芯片。通过多步液相光刻在两块玻璃载片之间生成微流控结构，省去了繁琐的键合步骤或昂贵的母版结构。利用新型无间隔剂的聚乙二醇二甲基丙烯酸酯（PEG-DA）光致聚合物方法，生成了高度仅为 25μm 的亲水通道的微流控器件。微流控芯片的特点是电极通道和分离床之间以及亲水表面具有离子可渗透的隔离壁。通过荧光吖嗪染料和荧光标记氨基酸的自由流电泳分离来证明芯片的性能。
