Imunofisiologia of Laboratory, Federal University of Maranhão, São Luís, MA, Brazil.
Toxicon. 2011 Nov;58(6-7):480-5. doi: 10.1016/j.toxicon.2011.08.006. Epub 2011 Aug 27.
Despite several studies showed that the Tityus serrulatus scorpion venom (Tsv) induces an inflammatory response, just a few have investigated the effect of the venom on the immune response. Therefore, the aim of this study was to evaluate alterations of venom application on lymphoid organs and on the recruitment and activation of cells and also on the cytokine production. Swiss male mice (2-3 months, 20-25 g) received a non-lethal dose of crude Tsv (200 μg/kg), diluted in sterile PBS by subcutaneous route. Control animals received only sterile PBS. The animals were sacrificed after 30, 120 and 360 min. The inflammatory parameters studied were skin histology at the site of venom application, leukocyte count, and blood cytokine levels (IL-6, IL-10, and TNF-α). Inguinal lymph node, spleen and bone marrow cellularity was determined for evaluation of the Tsv effect on immune system organs. The results showed that Tsv caused no local inflammation, but it induced an increase of blood neutrophils and serum IL-6, TNF-α and IL-10. After 360 min of envenomation there was a reduction in the cells number from peritoneum and spleen, but there was an increase in the cell number from lymph nodes. In conclusion, the Tsv induces systemic alterations characterized by changes in the cell number in lymphoid organs, increase pro and anti-inflammatory cytokines.
尽管有几项研究表明，特提斯 serrulatus 蝎毒液（Tsv）会引起炎症反应，但只有少数研究调查了毒液对免疫反应的影响。因此，本研究旨在评估毒液应用对淋巴器官的改变，以及对细胞募集和激活的影响，以及细胞因子的产生。雄性瑞士小鼠（2-3 个月，20-25 克）接受了非致死剂量的粗 Tsv（200μg/kg），通过皮下途径稀释在无菌 PBS 中。对照动物仅接受无菌 PBS。在 30、120 和 360 分钟后处死动物。研究的炎症参数包括毒液应用部位的皮肤组织学、白细胞计数以及血液细胞因子水平（IL-6、IL-10 和 TNF-α）。测定腹股沟淋巴结、脾脏和骨髓细胞数，以评估 Tsv 对免疫系统器官的影响。结果表明，Tsv 不会引起局部炎症，但会引起血液中性粒细胞和血清 IL-6、TNF-α 和 IL-10 的增加。在毒液注射 360 分钟后，腹膜和脾脏的细胞数量减少，但淋巴结的细胞数量增加。总之，Tsv 会引起全身改变，特征为淋巴器官中细胞数量的变化，增加促炎和抗炎细胞因子。
