School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
Int J Biol Macromol. 2011 Dec 1;49(5):1007-11. doi: 10.1016/j.ijbiomac.2011.08.024. Epub 2011 Aug 27.
Today more and more attentions had been attracted by many nutritionists and pharmacologists on polysaccharides from natural plants or animals due to their significant biological activities. In this research three polysaccharides (BRR-W1, BRR-WA1 and BRR-WA2) were isolated and purified from the water extract of Boschniakia rossica by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Chemical and physical characteristics of three polysaccharides were investigated by a combination of chemical and instrumental analysis methods. The assays of their effect on macrophages activation were also investigated in vitro, including phagocytosis of macrophages, detections for NO production and TNF-α secretion. The results indicated that the effect of polysaccharides on macrophages activation was influenced by their respective physicochemical properties.
如今，由于天然植物或动物来源的多糖具有显著的生物活性，越来越多的营养学家和药理学家开始关注它们。本研究采用 DEAE Sepharose Fast Flow 和 Sepharose 6 Fast Flow 柱层析法从肉苁蓉水提物中分离纯化得到三种多糖（BRR-W1、BRR-WA1 和 BRR-WA2）。采用化学和仪器分析相结合的方法研究了三种多糖的理化特性，并在体外考察了它们对巨噬细胞激活的作用，包括巨噬细胞的吞噬作用、NO 产生和 TNF-α分泌的检测。结果表明，多糖对巨噬细胞激活的影响与其理化性质有关。
