Department of Genetics and Biochemistry, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
Cell Immunol. 2011;271(2):329-34. doi: 10.1016/j.cellimm.2011.07.011. Epub 2011 Aug 25.
Astragalus polysaccharide (APS) was obtained by hot water extraction, alcohol precipitation, gel-permeation chromatography and ultrafiltration. Fluorescence material 2-aminoacridone (2-AMAC) labeled APS bind to macrophage in a time- dependent manner and the binding can be remarkably inhibited by APS. Furthermore, the effect of APS on RAW264.7 macrophage demonstrated APS increase the level of cytokines including TNF-α, GM-CSF and the production of NO. NF-κB protein levels are increased in response to APS. Blocking NF-κB with specific inhibitor resulted in decreased levels of NO and TNF-α. The results suggested that APS possess potent immunomodulatory activity by stimulating macrophage and could be used as an immunotherapeutic adjuvant.
黄芪多糖（APS）通过热水提取、醇沉、凝胶渗透色谱和超滤获得。荧光物质 2-氨基吖啶酮（2-AMAC）标记的 APS 与巨噬细胞以时间依赖的方式结合，并且这种结合可以被 APS 显著抑制。此外，APS 对 RAW264.7 巨噬细胞的作用表明 APS 增加了包括 TNF-α、GM-CSF 和 NO 产生在内的细胞因子水平。NF-κB 蛋白水平响应 APS 而增加。用特异性抑制剂阻断 NF-κB 导致 NO 和 TNF-α水平降低。结果表明，APS 通过刺激巨噬细胞具有强大的免疫调节活性，可作为免疫治疗佐剂使用。
