金雀异黄素对人永生化角质形成细胞中 UVB 诱导的光毒性的光保护作用。
Photoprotective effect of Calluna vulgaris extract against UVB-induced phototoxicity in human immortalized keratinocytes.
机构信息
Ion Chiricuta Cancer Institute, Cluj-Napoca, Romania.
出版信息
J Environ Pathol Toxicol Oncol. 2011;30(4):323-31. doi: 10.1615/jenvironpatholtoxicoloncol.v30.i4.50.
There is an increasing interest in the use of natural antioxidants as photoprotective agents against skin damages produced by ultraviolet radiation. The aim of our study was to investigate the protective effect of a Calluna vulgaris extract in human keratinocytes (HaCaT) exposed to ultraviolet B (UVB) radiation. HaCaT cells were treated with C. vulgaris extract 30 minutes prior to irradiation with UVB. The protective effect was evaluated by assessing cell viability using tetrasolium salt (MTT) assay; the generation of lipid peroxides was evaluated using malondialdehide assay (MDA); and DNA damage was evaluated using the comet assay and the quantification by ELISA of specific DNA photolesions [i.e., cyclobutane-pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs)]. After irradiation with cytotoxic doses of UVB (300 and 500 mJ/cm(2)), HaCaT cells pretreated with C. vulgaris extract (50 μg GAE/ml) showed significantly increased viability compared to control cells exposed to UVB only. Irradiation alone increased MDA levels in a dose-dependent fashion. Pretreatment with 12 μg GAE/ml extract lowered MDA levels both at 100 mJ/cm(2) (ρ<0.01) and 300 mJ/cm(2) (ρ<0.001). Treatment with C. vulgaris extract before exposure to UVB also reduced DNA damage: Lesion scores in a comet assay were significantly reduced at UVB doses of 50 mJ/cm2 (ρ<0.01) and 100 mJ/cm(2) (ρ<0.05), while CPDs and 6-4PPs (via ELISA) were significantly lower after irradiation with 100 mJ/cm(2) in the protected cells (ρ<0.05 for CPDs and ρ<0.001 for 6-4PPs). These results recommend the use of the C. vulgaris extract as photoprotective agent, in combination with sunscreens and/or other natural products with similar or complementary properties.
人们越来越关注使用天然抗氧化剂作为光保护剂，以防止紫外线辐射对皮肤造成损害。我们的研究目的是研究欧洲石楠提取物（Calluna vulgaris extract）对暴露于中波紫外线（UVB）辐射的人角质形成细胞（HaCaT）的保护作用。HaCaT 细胞在用 UVB 辐射照射前用 C. vulgaris 提取物处理 30 分钟。通过四唑盐（MTT）测定法评估细胞活力来评估保护作用；通过丙二醛测定法（MDA）评估脂质过氧化的产生；通过彗星试验评估 DNA 损伤，并通过 ELISA 定量测定特定的 DNA 光损伤[即环丁烷嘧啶二聚体（CPDs）和 6-4 光产物（6-4PPs）]。在用细胞毒性剂量的 UVB（300 和 500 mJ/cm2）照射后，与仅暴露于 UVB 的对照细胞相比，用 C. vulgaris 提取物（50 μg GAE/ml）预处理的 HaCaT 细胞显示出显著增加的活力。单独照射会以剂量依赖性方式增加 MDA 水平。用 12 μg GAE/ml 提取物预处理可降低 100 mJ/cm2（ρ<0.01）和 300 mJ/cm2（ρ<0.001）时的 MDA 水平。在用 UVB 照射之前用 C. vulgaris 提取物处理也会减少 DNA 损伤：在彗星试验中，在 UVB 剂量为 50 mJ/cm2（ρ<0.01）和 100 mJ/cm2（ρ<0.05）时，损伤评分显着降低，而在用 100 mJ/cm2 照射时，用保护细胞中的 CPDs 和 6-4PPs（通过 ELISA）显着降低（CPDs 的 ρ<0.05 和 6-4PPs 的 ρ<0.001）。这些结果建议将欧洲石楠提取物用作光保护剂，与防晒霜和/或具有类似或互补特性的其他天然产物结合使用。
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