Department of Nursing, Division of Basic Medical Sciences, Chronic Diseases and Health Promotion Research Center, Chang Gung Institute of Technology, Chia-Yi, Taiwan.
Int J Mol Sci. 2013 Feb 12;14(2):3860-73. doi: 10.3390/ijms14023860.
Administration of antioxidants and anti-inflammatory agents is an effective strategy for preventing ultraviolet (UV) irradiation-induced skin damage. Artocarpus communis possesses several pharmacological activities, such as antioxidant, anticancer and anti-inflammation. However, the photoprotective activity of methanol extract of A. communis heartwood (ACM) in ultraviolet irradiation-induced skin damage has not yet been investigated. The present study was performed using ultraviolet absorption, histopathological observation, antioxidant and anti-inflammation assays to elucidate the mechanism of the photoprotective activity of ACM. Our results indicated that ACM displayed a UVA and UVB absorption effect and then effectively decreased scaly skin, epidermis thickness and sunburn cells during ultraviolet irradiation in hairless mice. ACM not only decreased ultraviolet irradiation-mediated oxidative stress, including lowering the overproduction of reactive oxygen species and lipid peroxidation (p < 0.05), but also reduced the levels of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin 1β. Additionally, ACM can decrease the synthesis of cytosolic phospholipase A2, cyclooxygenase, inducible nitric oxide synthase and vascular cell adhesion molecular-1 via inhibiting TNF-α-independent pathways (p < 0.05) in UVB-mediated inflammation and formation of sunburn cells. Consequently, we concluded that ACM extract has a photoprotective effect against UVB-induced oxidative stress and inflammation due to its sunscreen property, and its topical formulations may be developed as therapeutic and/or cosmetic products in further studies.
抗氧化剂和抗炎药物的管理是预防紫外线（UV）照射引起的皮肤损伤的有效策略。菠萝蜜具有多种药理活性，如抗氧化、抗癌和抗炎作用。然而，菠萝蜜心材甲醇提取物（ACM）在紫外线照射诱导的皮肤损伤中的光保护活性尚未得到研究。本研究采用紫外线吸收、组织病理学观察、抗氧化和抗炎测定法，阐明了 ACM 的光保护活性机制。我们的结果表明，ACM 显示出 UVA 和 UVB 吸收作用，然后在无毛小鼠的紫外线照射下有效减少鳞屑、表皮厚度和晒伤细胞。ACM 不仅降低了紫外线照射介导的氧化应激，包括降低活性氧和脂质过氧化的过度产生（p<0.05），还降低了促炎细胞因子，包括肿瘤坏死因子-α（TNF-α）和白细胞介素 1β的水平。此外，ACM 可以通过抑制 TNF-α 非依赖性途径（p<0.05）降低细胞质磷脂酶 A2、环氧化酶、诱导型一氧化氮合酶和血管细胞黏附分子-1的合成，从而减轻 UVB 介导的炎症和晒伤细胞的形成。因此，我们得出结论，由于 ACM 提取物具有防晒性能，因此对 UVB 诱导的氧化应激和炎症具有光保护作用，其局部制剂可能在进一步的研究中开发为治疗和/或化妆品产品。
