McGowan M K, Andrews K M, Kelly J, Grossman S P
Committee on Biopsychology, University of Chicago, Illinois 60643.
Behav Neurosci. 1990 Apr;104(2):373-85. doi: 10.1037//0735-7044.104.2.373.
In Experiment 1, rats were chronically infused with insulin (2.7, 27, or 270 ng/hr) or 0.9% saline into the ventromedial (VMH), medial perifornical (PF), or lateral (LH) hypothalamus. VMH infusions of insulin caused a significant, dose-dependent decrease in food intake and body weight; PF infusion of insulin was less effective, but significant; whereas LH infusions of insulin were ineffective. In Experiment 2, rats were chronically infused with insulin (0.54 ng/hr) or 0.9% saline into the VMH, paraventricular (PVN), or posterior (PN) hypothalamic nucleus. Subjects that received VMH or PN infusions of insulin failed to regain weight lost as a result of surgery even 2 weeks after infusion; subjects that received PVN infusions of insulin regained their preoperative weights faster than did controls. All of the groups that received insulin significantly increased their daytime food intake during the infusion period and decreased their night food intake slightly; 24-hr food intake remained unchanged.
在实验1中，向大鼠的腹内侧下丘脑（VMH）、穹窿周内侧区（PF）或外侧下丘脑（LH）长期输注胰岛素（2.7、27或270纳克/小时）或0.9%生理盐水。向VMH输注胰岛素导致食物摄入量和体重显著的剂量依赖性下降；向PF输注胰岛素效果稍差，但也显著；而向LH输注胰岛素则无效。在实验2中，向大鼠的VMH、室旁核（PVN）或下丘脑后核（PN）长期输注胰岛素（0.54纳克/小时）或0.9%生理盐水。接受VMH或PN输注胰岛素的受试大鼠即使在输注后2周仍未能恢复因手术而减轻的体重；接受PVN输注胰岛素的受试大鼠比对照组更快地恢复到术前体重。所有接受胰岛素输注的组在输注期间白天食物摄入量显著增加，夜间食物摄入量略有减少；24小时食物摄入量保持不变。
