Federal Research Institute for Animal Health, Naumburger Strasse 96a, 07743 Jena, Germany.
Vet Rec. 2011 Nov 5;169(19):495. doi: 10.1136/vr.d5410. Epub 2011 Sep 6.
The sensitivity and specificity of three commercially available complement fixation test (CFT) antigens from c.c.pro (c.c.pro), Central Veterinary Institute of Wageningen UR (CIDC) and the United States Department of Agriculture (USDA) were comparatively evaluated by testing 410 sera collected from glanders-endemic and non-endemic areas (200 true-negative randomly collected sera and 210 sera collected from experimentally immunised animals (12 rabbits, 19 horses), clinically positive (135) and culture-positive (44) horses, donkeys and mules). Immunoblotting (IB) was used as the gold standard test. Highest sensitivity was shown for the CIDC antigen (100 per cent) followed by the c.c.pro antigen (99.39 per cent). However, the USDA antigen showed substantially less (p<0.05) sensitivity (62.19 per cent). Highest specificity was found for the USDA antigen (100 per cent) followed by the CIDC (97.5 per cent) and c.c.pro antigen (96.5 per cent). Positive and negative predictive values (assumed glanders prevalence of <0.1 per cent) for each antigen were calculated to be 95.88 and 99.48 (c.c.pro), 97.04 and 100 (CIDC), 100 and 76.33 per cent (USDA), respectively. Almost perfect agreement (0.96) was found between CFT using either c.c.pro or CIDC and IB.
三种市售补体固定试验（CFT）抗原（c.c.pro、CIDC 和 USDA）的敏感性和特异性比较评估，使用从流行和非流行地区（200 份随机采集的真阴性血清和 210 份来自实验免疫动物的血清（12 只兔、19 匹马））采集的 410 份血清进行检测。免疫印迹（IB）被用作金标准检测方法。CIDC 抗原的敏感性最高（100%），其次是 c.c.pro 抗原（99.39%）。然而，USDA 抗原的敏感性明显较低（p<0.05）（62.19%）。USDA 抗原的特异性最高（100%），其次是 CIDC（97.5%）和 c.c.pro 抗原（96.5%）。每种抗原的阳性和阴性预测值（假设马鼻疽的流行率<0.1%）分别计算为 95.88%和 99.48%（c.c.pro）、97.04%和 100%（CIDC）、100%和 76.33%（USDA）。使用 c.c.pro 或 CIDC 进行的 CFT 与 IB 之间存在几乎完美的一致性（0.96）。
