Elschner Mandy Carolina, Melzer Falk, Singha Harisankar, Muhammad Saqib, Gardner Ian, Neubauer Heinrich
Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
Indian Council of Agricultural Research, National Research Centre on Equines, Hisar, India.
Front Vet Sci. 2021 Feb 16;8:628389. doi: 10.3389/fvets.2021.628389. eCollection 2021.
Glanders, caused by (.) is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement.
鼻疽是由鼻疽杆菌引起的马属动物法定人畜共患病。对于国际贸易和马属动物的移动，需要出具针对鼻疽杆菌抗体的血清学检测阴性结果证书。迄今为止，补体结合试验（CFT）是出具这些健康证书的强制检测方法。CFT难以标准化，且由于其特异性较差，常导致假阳性反应，从而造成贸易限制并带来相当大的经济后果。在本研究中，使用400份马属动物阴性和370份鼻疽阳性现场样本对新型ID Screen鼻疽双抗原多物种ELISA（GLANDA - ELISA）（IDvet公司，法国格拉贝）进行了评估。GLANDA - ELISA的特异性（99.8%）显著高于CFT（97.0%）。考虑到CFT（96.5%）和ELISA（98.1%）相当的敏感性，这种新型GLANDA - ELISA检测似乎是一种合适的确诊检测方法，也是用于马匹贸易或移动血清学检测的切实可行的替代方法。
