González Felix Diaz, Muiño Rodrigo, Pereira Víctor, Campos Rómulo, Benedito José Luis
Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
J Vet Sci. 2011 Sep;12(3):251-5. doi: 10.4142/jvs.2011.12.3.251.
Blood indicators are used as a tool to diagnose metabolic disorders. The present work was conducted to study the relationships among blood indicators of lipomobilization and hepatic function in high-yielding dairy cows. Two groups of Holstein cows were studied: 27 early lactation cows and 14 mid lactation cows from four different herds with similar husbandry characteristics in Galicia, Spain. Blood samples were obtained to measure beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase. Cows in early lactation had higher levels of BHB and NEFA than mid lactation cows. High lipomobilization (NEFA > 400 µmol/L) was detected in 67% and 7% of early lactation and mid lactation cows, respectively, while subclinical ketosis (BHB > 1.2 mmol/L) was detected in 41% and 28% of the early lactation and lactation cows, respectively. TG concentrations were low in all cows suffering subclinical ketosis and in 61% of the cows with high lipomobilization. During early lactation, 30% of cows suffered hepatic lipidosis as detected by levels of AST. Compromised hepatic function was observed in early lactation cows as shown by lower concentrations of glucose, total protein, and urea.
血液指标被用作诊断代谢紊乱的工具。本研究旨在探讨高产奶牛脂肪动员血液指标与肝功能之间的关系。研究了两组荷斯坦奶牛：来自西班牙加利西亚四个具有相似饲养特征牛群的27头初产奶牛和14头中产奶牛。采集血样以测量β-羟基丁酸（BHB）、非酯化脂肪酸（NEFA）、甘油三酯（TG）以及天冬氨酸转氨酶（AST）和γ-谷氨酰转移酶的活性。初产奶牛的BHB和NEFA水平高于中产奶牛。分别在67%的初产奶牛和7%的中产奶牛中检测到高脂肪动员（NEFA>400µmol/L），而分别在41%的初产奶牛和28%的泌乳奶牛中检测到亚临床酮病（BHB>1.2mmol/L）。所有患亚临床酮病的奶牛以及61%高脂肪动员的奶牛TG浓度较低。在初产期间，通过AST水平检测到30%的奶牛患肝脂肪变性。初产奶牛出现肝功能受损，表现为葡萄糖、总蛋白和尿素浓度较低。
