Ashri Nadia Y, Abdel-Rehim Mohamed
Najd Consulting Hospital, Riyadh, Kingdom of Saudi Arabia.
Bioanalysis. 2011 Sep;3(17):2003-18. doi: 10.4155/bio.11.201.
The importance of sample preparation methods as the first stage in bioanalysis is described. In this article, the sample preparation concept and strategies will be discussed, along with the requirements for good sample preparation. The most widely used sample preparation methods in the pharmaceutical industry are presented; for example, the need for same-day rotation of results from large numbers of biological samples in pharmaceutical industry makes high throughput bioanalysis more essential. In this article, high-throughput sample preparation techniques are presented; examples are given of the extraction and concentration of analytes from biological matrices, including protein precipitation, solid-phase extraction, liquid-liquid extraction and microextraction-related techniques. Finally, the potential role of selective extraction methods, including molecular imprinted phases, is considered.
描述了样品制备方法作为生物分析第一阶段的重要性。在本文中，将讨论样品制备的概念和策略，以及良好样品制备的要求。介绍了制药行业中最广泛使用的样品制备方法；例如，制药行业对大量生物样品结果进行当日周转的需求使得高通量生物分析更为重要。本文介绍了高通量样品制备技术；给出了从生物基质中提取和浓缩分析物的示例，包括蛋白沉淀、固相萃取、液液萃取和微萃取相关技术。最后，考虑了包括分子印迹相在内的选择性萃取方法的潜在作用。
