Institute of Food Sciences, National Research Council (CNR), Avellino, Italy.
J Appl Microbiol. 2011 Dec;111(6):1482-91. doi: 10.1111/j.1365-2672.2011.05147.x. Epub 2011 Oct 4.
This study was performed to ascertain the immunomodulatory effect of Lactobacillus paracasei strains. These strains were also genetically characterized.
The strains were genetically differentiated by using the fluorescent-amplified fragment length polymorphism technique, which led to the identification of several molecular markers unique to each strain. To determine the immunomodulatory properties, we evaluated the effect of strains on dendritic cell maturation, dextran uptake, ability to induce proliferation of allogenic T cells and cytokine secretion. The results indicated that all the strains stimulated phenotypic maturation of dendritic cells (DCs), but they acted differently on DCs in relation to the other tested properties; notably, a different effect on cytokine secretion was detected.
The results of this study revealed different immunomodulatory properties of strains of the species Lact. paracasei. Strain IMPC 4.1 showed an interesting anti-inflammatory ability. Probiotic strains IMPC 2.1 and LMG P-17806 were characterized by a similar and intermediate ability to induce cytokine secretion in contrast to the very low ability of strain LMG 23554.
Our results confirm that each single strain of a bacterial species appears to influence the immune system in a peculiar manner. The evaluation of the different types and/or levels of cytokines whose secretion is induced by each strain could be relevant to define its pro- or anti-inflammatory properties and its more appropriate clinical use.
本研究旨在确定副干酪乳杆菌菌株的免疫调节作用。这些菌株也进行了遗传特征分析。
使用荧光扩增片段长度多态性技术对菌株进行遗传分化，从而鉴定出每种菌株特有的几个分子标记。为了确定免疫调节特性，我们评估了菌株对树突状细胞成熟、葡聚糖摄取、诱导同种异体 T 细胞增殖和细胞因子分泌的影响。结果表明，所有菌株均刺激树突状细胞（DC）的表型成熟，但在其他测试特性方面，对 DC 的作用方式不同；值得注意的是，检测到细胞因子分泌的不同影响。
本研究结果揭示了副干酪乳杆菌属菌株的不同免疫调节特性。菌株 IMPC 4.1 表现出有趣的抗炎能力。益生菌菌株 IMPC 2.1 和 LMG P-17806 表现出相似且中等的诱导细胞因子分泌能力，而菌株 LMG 23554 的能力则非常低。
我们的结果证实，每种细菌物种的单一菌株似乎以独特的方式影响免疫系统。评估每种菌株诱导分泌的不同类型和/或水平的细胞因子可能与其抗炎或促炎特性及其更合适的临床应用相关。
