Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Amendola 122/O, Bari 70126, Italy.
Institute of Food Sciences, National Research Council (CNR), Via Roma 52, Avellino 83100, Italy.
Nutrients. 2016 Oct 14;8(10):635. doi: 10.3390/nu8100635.
The most recent trend in research on probiotic bacteria aims at the exploitation of bioactive bacterial compounds that are responsible for health-promoting effects and suitable for medical applications. Therefore, the main purpose of this study was to ascertain if the immunomodulatory effects of strains on dendritic cells (DCs) were caused by bacterial metabolites released in the culture medium. For that reason, bacterial strains were grown in two media generally used for the culture of DCs, and the effects of culture filtrates on the maturation of DCs and cytokine production were evaluated. Moreover, to reveal potential synergistic effects on the immunomodulation of DCs, an artichoke phenolic extract (APE) was added to the media before bacterial growth. The experiments pointed out an interesting anti-inflammatory activity of a culture filtrate obtained after growing a probiotic strain in one of the media supplemented with APE. Therefore, this culture filtrate-which combines the anti-inflammatory activity and the other well-known health-promoting properties of artichoke phenolic compounds-could represent the basis for future particular exploitations.
目前，益生菌细菌研究的最新趋势旨在开发具有促进健康作用且适用于医疗应用的生物活性细菌化合物。因此，本研究的主要目的是确定菌株对树突状细胞 (DC) 的免疫调节作用是否是由培养基中释放的细菌代谢物引起的。为此，将细菌菌株在两种通常用于培养 DC 的培养基中生长，并评估培养滤液对 DC 成熟和细胞因子产生的影响。此外，为了揭示对 DC 免疫调节的潜在协同作用，在细菌生长前将朝鲜蓟酚类提取物 (APE) 添加到培养基中。实验指出，在用 APE 补充的一种培养基中生长的益生菌 菌株的培养滤液具有有趣的抗炎活性。因此，这种培养滤液结合了朝鲜蓟酚类化合物的抗炎活性和其他众所周知的促进健康的特性，可能成为未来特殊开发的基础。
