酵母中 DNA 复制的动力学。
Dynamics of DNA replication in yeast.
机构信息
Centre for Genetics and Genomics, University of Nottingham, United Kingdom.
出版信息
Phys Rev Lett. 2011 Aug 5;107(6):068103. doi: 10.1103/PhysRevLett.107.068103. Epub 2011 Aug 4.
Abstract
We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae.
摘要
我们提出了一个 DNA 复制的空间动力学数学模型。利用这个模型，我们确定了每个染色体位置复制的时间概率分布。由此我们表明，与之前的报告相反，平均复制时间曲线不能直接用于确定起始点参数。我们证明了复制动力学的随机性在实验测量的群体平均数据中留下了明显的特征，并且我们表明可以从这些数据推断出激活时间概率分布的宽度。我们的结果与酿酒酵母的实验测量结果相比具有优势。
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