Laboratory of Experimental Cell Culture, Department of Endodontics, Fluminense Federal University (UFF), Niteroi, RJ. Brazil.
Int Endod J. 2012 Jan;45(1):12-8. doi: 10.1111/j.1365-2591.2011.01941.x. Epub 2011 Sep 8.
To compare the cytotoxicity of four endodontic sealers (Sealapex, Pulp Canal Sealer EWT, Real Seal and MTA Fillapex) either 1 or 7 days after mixing, when assessed through a multiparametric analysis employing human primary cells closely related to periapical tissues.
Extracts of each sealer were prepared following 24-h exposure to culture media, at either 24 h or 7 days after mixing. Primary human osteoblasts were exposed to extracts for 24 h, at 37 °C with 5% CO(2) , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). Results from each test and experimental time were compared by 2-way analysis of variance (anova).
All endodontic sealers had strong cytotoxicity 24 h after mixing, according to all parameters evaluated. At a longer setting period (7 days), viability for Sealapex was significantly increased (P < 0.05) and Pulp Canal Sealer achieved levels of cytocompatibility similar to the control group. The anova indicated a general correlation between the cytotoxicity of the materials and the time after mixing, with some level of dependence on the cell viability assay employed.
All materials had high cytotoxic levels for human primary cells, mostly on a time-dependent basis, as shown by three different cell viability tests.
通过使用与人根尖周组织密切相关的原代细胞进行多参数分析，比较 4 种根管封闭剂（Sealapex、Pulp Canal Sealer EWT、Real Seal 和 MTA Fillapex）在混合后 1 天或 7 天后的细胞毒性。
在混合后 24 小时或 7 天后，将每种封闭剂的提取物暴露于培养基中 24 小时。将原代人成骨细胞暴露于提取物中 24 小时，在 37°C 和 5%CO2 下，通过多参数测定法评估细胞活力，该测定法依次评估同一细胞的线粒体活性（XTT）、膜完整性（中性红试验）和总细胞密度（结晶紫染料排除试验）。通过双因素方差分析（anova）比较每个试验和实验时间的结果。
所有根管封闭剂在混合后 24 小时均具有很强的细胞毒性，根据所有评估的参数。在较长的凝固时间（7 天）后，Sealapex 的活力显著增加（P<0.05），Pulp Canal Sealer 达到了与对照组相似的细胞相容性水平。anova 表明，材料的细胞毒性与混合后的时间之间存在一般相关性，与所使用的细胞活力测定法有一定的依赖性。
所有材料对人原代细胞均具有高细胞毒性水平，主要是基于时间依赖性，通过 3 种不同的细胞活力测定法显示。
