Food and Biobased Research, Wageningen University and Research Centre, Wageningen, The Netherlands.
Anal Bioanal Chem. 2012 Jan;402(2):593-600. doi: 10.1007/s00216-011-5340-5. Epub 2011 Sep 9.
Carbon nanoparticles (CNPs) labeled with reporter molecules can serve as signaling labels in rapid diagnostic assays as an alternative to gold, colored latex, silica, quantum dots, or up-converting phosphor nanoparticles. Detailed here is the preparation of biomolecule-labeled CNPs and examples of their use as a versatile label. CNPs can be loaded with a range of biomolecules, such as DNA, antibodies, and proteins (e.g., neutravidin or a fusion protein of neutravidin with an enzyme), and the resulting conjugates can be used to detect analytes of high or low molecular mass.
碳纳米粒子（CNPs）标记有报告分子，可以作为金、彩色乳胶、二氧化硅、量子点或上转换磷纳米粒子的替代物，用于快速诊断测定中的信号标记。本文详细介绍了生物分子标记的 CNPs 的制备方法及其作为通用标记的应用实例。CNPs 可以负载多种生物分子，如 DNA、抗体和蛋白质（如亲和素或亲和素与酶的融合蛋白），所得缀合物可用于检测高或低分子量的分析物。
