Division of Biochemistry & Biotechnology, National Centre for Disease Control 22, Sham Nath Marg, Delhi, India.
Epidemiol Infect. 2012 Jul;140(7):1337-42. doi: 10.1017/S0950268811001671. Epub 2011 Sep 12.
Independent outbreaks of dengue virus (DENV) infection and sporadic cases of chikungunya virus (CHIKV) have been recorded in the metropolitan city of Delhi on several occasions in the past. However, during a recent 2010 arboviral outbreak in Delhi many cases turned negative for DENV. This prompted us to use duplex reverse transcriptase-polymerase chain reaction (D-RT-PCR) to establish the aetiology of dengue/chikungunya through sequencing of CprM and E1 genes of dengue and chikungunya viruses. Interestingly, for the first time, both DENV and CHIKV co-circulated simultaneously and in equally dominant proportion during the post-monsoon period of 2010. DENV-1 genotype III and the East Central South African genotype of CHIKV were associated with post-monsoon spread of these viruses.
在过去的几次中，德里的大都市都曾记录到登革热病毒（DENV）感染的独立爆发和散发性基孔肯雅热病毒（CHIKV）病例。然而，在德里最近的 2010 年虫媒病毒爆发期间，许多病例的登革热病毒检测呈阴性。这促使我们使用双重逆转录-聚合酶链反应（D-RT-PCR）通过对登革热和基孔肯雅热病毒的 CprM 和 E1 基因进行测序来确定登革热/基孔肯雅热的病因。有趣的是，这是第一次在 2010 年的后季风期同时并且以同样占主导地位的比例共同循环传播 DENV 和 CHIKV。与这些病毒在后季风期传播相关的是 DENV-1 基因型 III 和中东南非的 CHIKV 基因型。
