Department of Pediatrics, Division of Neonatology, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
J Pediatr. 2012 Feb;160(2):245-51. doi: 10.1016/j.jpeds.2011.07.040. Epub 2011 Sep 9.
To measure the circulating concentrations of nitric oxide (NO) adducts with NO bioactivity after inhaled NO (iNO) therapy in infants with pulmonary hypertension.
In this single center study, 5 sequential blood samples were collected from infants with pulmonary hypertension before, during, and after therapy with iNO (n = 17). Samples were collected from a control group of hospitalized infants without pulmonary hypertension (n = 16) and from healthy adults for comparison (n = 12).
After beginning iNO (20 ppm) whole blood nitrite levels increased approximately two-fold within 2 hours (P<.01). Whole blood nitrate levels increased to 4-fold higher than baseline during treatment with 20 ppm iNO (P<.01). S-nitrosohemoglobin increased measurably after beginning iNO (P<.01), whereas iron nitrosyl hemoglobin and total hemoglobin-bound NO-species compounds did not change.
Treatment of pulmonary hypertensive infants with iNO results in increases in levels of nitrite, nitrate, and S-nitrosohemoglobin in circulating blood. We speculate that these compounds may be carriers of NO bioactivity throughout the body and account for peripheral effects of iNO in the brain, heart, and other organs.
测量吸入一氧化氮（iNO）治疗后肺动脉高压婴儿循环中具有生物活性的一氧化氮（NO）加合物的浓度。
在这项单中心研究中，从接受 iNO 治疗的肺动脉高压婴儿（n=17）治疗前、治疗中和治疗后采集了 5 个连续的血样。还从没有肺动脉高压的住院婴儿（n=16）和健康成年人（n=12）对照组中采集了样本进行比较。
开始 iNO（20ppm）后，全血亚硝酸盐水平在 2 小时内增加了约两倍（P<.01）。在 20ppm iNO 治疗期间，全血硝酸盐水平增加到基线的 4 倍以上（P<.01）。开始 iNO 后可测量到 S-亚硝基血红蛋白增加（P<.01），而铁亚硝基血红蛋白和总血红蛋白结合的 NO 种类化合物没有变化。
用 iNO 治疗患有肺动脉高压的婴儿会导致循环血液中亚硝酸盐、硝酸盐和 S-亚硝基血红蛋白水平升高。我们推测这些化合物可能是全身 NO 生物活性的载体，并解释了 iNO 在大脑、心脏和其他器官中的外周效应。
