Kim Ji Young, Choe Yong Beom, Ahn Kyu Joong, Lee Yang Won
Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
Ann Dermatol. 2011 Aug;23(3):304-12. doi: 10.5021/ad.2011.23.3.304. Epub 2011 Aug 6.
Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years.
To assess the value of multiplex PCR in identification of dermatophytes.
Using three specially-designed primers which contained the ITS1-2, 18S rRNA, and 28S rRNA regions, three cycles of PCR were performed on 11 standard strains and scales were collected from 73 patients with fungal infection.
The 11 standard strains were successfully identified with analysis of band patterns of ITS1-2, 18S rRNA, and 28S rRNA, obtained from PCR. Based on this information, the causative organisms in 73 patients with fungal infection were revealed to be T. rubrum in 69 cases, T. menta in 1 case, T. tonsurans in 2 cases, and M. gypseum in one case.
With three cycles of PCR using three sets of primers, 11 standard strains and the clinical strains from 73 patients with fungal infection were successfully identified.
多重聚合酶链反应（PCR）可使用两种以上引物扩增两种以上目标DNA分子。该方法对于在单个试管中同时检测多种其他生物体也很有用，近年来其应用范围在临床微生物学领域广泛扩大。
评估多重PCR在皮肤癣菌鉴定中的价值。
使用三种专门设计的包含ITS1-2、18S rRNA和28S rRNA区域的引物，对11株标准菌株和从73例真菌感染患者采集的鳞屑进行三轮PCR。
通过对PCR获得的ITS1-2、18S rRNA和28S rRNA条带模式分析，成功鉴定了11株标准菌株。基于此信息，73例真菌感染患者的致病生物体显示为红色毛癣菌69例、须癣毛癣菌1例、断发毛癣菌2例、石膏样小孢子菌1例。
使用三组引物进行三轮PCR，成功鉴定了11株标准菌株和73例真菌感染患者的临床菌株。
