Department of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto, Indonesia.
Bioprocess Biosyst Eng. 2012 Jan;35(1-2):93-8. doi: 10.1007/s00449-011-0608-x. Epub 2011 Sep 10.
Red seaweed, Kappaphycus alvarezii, holds great promise for use in biofuel production due to its high carbohydrate content. In this study, we investigated the effect of fermentation inhibitors to the K. alvarezii hydrolysate on cell growth and ethanol fermentation. In addition, detoxification of fermentation inhibitors was performed to decrease the fermentation inhibitory effect. 5-Hydroxymethylfurfural and levulinic acid, which are liberated from acidic hydrolysis, was also observed in the hydrolysate of K. alvarezii. These compounds inhibited ethanol fermentation. In order to remove these inhibitors, activated charcoal and calcium hydroxide were introduced. The efficiency of activated charcoals was examined and over-liming was used to remove the inhibitors. Activated charcoal was found to be more effective than calcium hydroxide to remove the inhibitors. Detoxification by activated charcoal strongly improved the fermentability of dilute acid hydrolysate in the production of bioethanol from K. alvarezii with Saccharomyces cerevisiae. The optimal detoxifying conditions were found to be below an activated charcoal concentration of 5%.
红海藻，卡拉胶，由于其高碳水化合物含量，在生物燃料生产中具有很大的应用潜力。在这项研究中，我们研究了发酵抑制剂对卡拉胶水解物对细胞生长和乙醇发酵的影响。此外，还进行了发酵抑制剂的解毒作用，以降低发酵抑制作用。从酸性水解中释放出来的 5-羟甲基糠醛和乙酰丙酸也在卡拉胶的水解物中被观察到。这些化合物抑制了乙醇发酵。为了去除这些抑制剂，引入了活性炭和氢氧化钙。考察了活性炭的效率，并采用过碱化来去除抑制剂。活性炭的去除效果优于氢氧化钙。活性炭的解毒作用大大提高了酿酒酵母从卡拉胶生产生物乙醇的稀酸水解物的发酵性能。发现最佳解毒条件为活性炭浓度低于 5%。
