Central Salt and Marine Chemicals Research Institute (CSIR), Gijubhai Badheka Marg, Bhavnagar 364 021, Gujarat, India.
Bioresour Technol. 2012 Jan;103(1):180-5. doi: 10.1016/j.biortech.2011.10.015. Epub 2011 Oct 13.
The present study describes production of bio-ethanol from fresh red alga, Kappaphycus alvarezii. It was crushed to expel sap--a biofertilizer--while residual biomass was saccharified at 100 °C in 0.9 N H2SO4. The hydrolysate was repeatedly treated with additional granules to achieve desired reducing sugar concentration. The best yields for saccharification, inclusive of sugar loss in residue, were 26.2% and 30.6% (w/w) at laboratory (250 g) and bench (16 kg) scales, respectively. The hydrolysate was neutralized with lime and the filtrate was desalted by electrodialysis. Saccharomyces cerevisiae (NCIM 3523) was used for ethanol production from this non-traditional bio-resource. Fermentation at laboratory and bench scales converted ca. 80% of reducing sugar into ethanol in near quantitative selectivity. A petrol vehicle was successfully run with E10 gasoline made from the seaweed-based ethanol. Co-production of ethanol and bio-fertilizer from this seaweed may emerge as a promising alternative to land-based bio-ethanol.
本研究描述了从新鲜红藻 Kappaphycus alvarezii 生产生物乙醇的过程。它被压碎以排出汁液——一种生物肥料——而剩余的生物质在 100°C 的 0.9N H2SO4 中糖化。水解物用额外的颗粒反复处理，以达到所需的还原糖浓度。在实验室（250 克）和台架（16 公斤）规模下，糖化的最佳收率，包括残渣中的糖损失，分别为 26.2%和 30.6%（w/w）。水解物用石灰中和，滤液用电渗析脱盐。非传统生物资源的乙醇生产采用酿酒酵母（NCIM 3523）。在实验室和台架规模的发酵中，约 80%的还原糖几乎定量地转化为乙醇。一辆汽油车成功地使用了由海藻基乙醇制成的 E10 汽油。从这种海藻中同时生产乙醇和生物肥料可能成为陆地生物乙醇的一种有前途的替代方案。
