Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Chin J Integr Med. 2011 Sep;17(9):691-7. doi: 10.1007/s11655-011-0847-3. Epub 2011 Sep 11.
To study the inhibitory effect of Fuzheng Yiliu Granule (FYG) on hepatocellular cancer (HCC) and investigate the mechanism mediating its bioactivity.
H22 tumor-bearing ICR mice were treated with FYG [3.6 g/(kg·d)] for 5 days. Tumor volume and tumor weight, percentages of CD3(+), CD4(+), CD8(+), and natural killer (NK) cells in peripheral blood, tumor apoptosis and serum levels of interleukin-2 (IL-2), and tumor necrosis factor-α (TNF-α) were evaluated. FYG-containing serum was prepared from SD rats treated for 7 days [high dose 3.6 g/(kg·d); middle dose 1.8 g/(kg·d); low dose 0.9 g/(kg·d)]. Cell cycle, cell viability, and apoptosis were evaluated after HepG2 cell line was cultured in FYG-containing serum for 48 h. The levels of IL-2 and TNF-α in FYG-containing serum were also determined.
FYG produced a potent antitumor effect (P<0.01) and induced marked apoptosis of the tumor tissue (P<0.05). Mice treated with FYG had higher percentages of CD3(+) and CD4(+) (P<0.05), and more NK cells (P<0.01) in the peripheral blood than those in the animals treated with normal saline. Mice receiving FYG had the highest serum levels of IL-2 and TNF-α (P<0.01). High-dose FYG-containing serum significantly decreased HepG2 cell viability, inhibited cell proliferation (P<0.05), and induced apoptosis (P<0.01). In addition, the levels of IL-2 and TNF-α of high-dose-containing serum were higher than the blank serum (P<0.01).
FYG could inhibit HCC growth by regulating immune function and inducing apoptosis of tumor cells in vivo and in vitro.
研究扶正抑瘤颗粒（FYG）对肝癌（HCC）的抑制作用，并探讨介导其生物活性的机制。
用 FYG [3.6 g/（kg·d）] 处理荷瘤 ICR 小鼠 5 天。评估肿瘤体积和肿瘤重量、外周血中 CD3(+)、CD4(+)、CD8(+)和自然杀伤（NK）细胞的百分比、肿瘤凋亡以及血清中白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）的水平。用 FYG 处理 SD 大鼠 7 天[高剂量 3.6 g/（kg·d）；中剂量 1.8 g/（kg·d）；低剂量 0.9 g/（kg·d）]制备 FYG 含药血清。用 FYG 含药血清培养 HepG2 细胞系 48 h 后，评估细胞周期、细胞活力和细胞凋亡。还测定 FYG 含药血清中 IL-2 和 TNF-α的水平。
FYG 产生了强大的抗肿瘤作用（P<0.01），并诱导肿瘤组织明显凋亡（P<0.05）。与生理盐水处理的动物相比，用 FYG 处理的小鼠外周血中 CD3(+)和 CD4(+)的百分比更高（P<0.05），NK 细胞更多（P<0.01）。接受 FYG 的小鼠血清中 IL-2 和 TNF-α水平最高（P<0.01）。高剂量 FYG 含药血清显著降低 HepG2 细胞活力，抑制细胞增殖（P<0.05），并诱导细胞凋亡（P<0.01）。此外，高剂量含药血清中 IL-2 和 TNF-α的水平高于空白血清（P<0.01）。
FYG 可通过调节免疫功能和诱导体内、体外肿瘤细胞凋亡来抑制 HCC 生长。
