Maule A G, Halton D W, Johnston C F, Shaw C, Fairweather I
School of Biology and Biochemistry, Queen's University of Belfast, Northern Ireland, UK.
Parasitol Res. 1990;76(5):409-19. doi: 10.1007/BF00933549.
The reproductive system of the monogenean gill parasite, Diclidophora merlangi, was examined for the presence of cholinergic, serotoninergic and peptidergic innervation using cytochemical and immunocytochemical techniques. Cholinesterase activity and 5-hydroxytryptamine immunoreactivity (5-HT-IR) were confined to neural elements of the male reproductive system, being evident in the innervation of the cirrus, whereas only 5-HT was present in nerves and somata of the elongate seminal vesicle. Peptidergic innervation was localised to both the male and female reproductive systems of the worm. Within the female reproductive apparatus pancreatic polypeptide, peptide tyrosine tyrosine, neuropeptide Y, substance P, neurokinin A, eledoisin, FMRFamide and gastrin/cholecystokinin immunoreactive fibres and somata were observed in the oviduct, vitelline reservoir and ovovitelline duct. Intense peptide immunoreactivity was identified in fibres in the wall of the ootype and in a surrounding population (greater than 100) of somata that were situated beyond Mehlis' gland cells and all of which were connected to the ootype wall by fine cytoplasmic connectives. The strategic location of this peptidergic cell population infers its involvement in the egg-forming sequence in this platyhelminth parasite.
利用细胞化学和免疫细胞化学技术，对单殖吸虫鳃寄生虫梅氏双盘吸虫（Diclidophora merlangi）的生殖系统进行检查，以确定是否存在胆碱能、5-羟色胺能和肽能神经支配。胆碱酯酶活性和5-羟色胺免疫反应性（5-HT-IR）仅限于雄性生殖系统的神经成分，在阴茎的神经支配中很明显，而在细长精囊的神经和胞体中仅存在5-羟色胺。肽能神经支配定位于该蠕虫的雄性和雌性生殖系统。在雌性生殖器官内，在输卵管、卵黄囊和卵黄管中观察到胰多肽、肽酪氨酸酪氨酸、神经肽Y、P物质、神经激肽A、伊勒多辛、FMRF酰胺和胃泌素/胆囊收缩素免疫反应性纤维和胞体。在卵模壁的纤维以及位于梅氏腺细胞之外的一群（超过100个）胞体中发现了强烈的肽免疫反应性，所有这些胞体都通过细细胞质连接与卵模壁相连。这群肽能细胞的关键位置表明其参与了这种扁形虫寄生虫的卵子形成过程。
