CIGMH, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
Nanotechnology. 2011 Oct 14;22(41):415202. doi: 10.1088/0957-4484/22/41/415202. Epub 2011 Sep 14.
We propose an experimental-based tool for dealing with fluorescence modulation close to nanoparticles for application in studies of fluorophores in the vicinity of gold nanoparticles (AuNPs), typically addressed via theoretical models. We performed a photophysical characterization of fluorophores in the vicinity of AuNPs, showing that correct Φ(F) determination suffers from a local pH effect, and address the observed radiative enhancement. Our approach is based on the experimental assurance that the reference fluorophores are in the same optical conditions as those of the AuNP-fluorophore conjugates. We demonstrate the relevance for introducing corrections for the inner filter effect and the reabsorption of the emitted light caused by AuNPs. The proposed approach could circumvent the need for theoretical based corrections and allow for more accurate determination of fluorescence emission in the vicinity of gold nanoparticles.
我们提出了一种基于实验的工具，用于处理接近纳米粒子的荧光调制，以应用于金纳米粒子（AuNPs）附近荧光团的研究，通常通过理论模型来解决。我们对 AuNPs 附近荧光团的光物理特性进行了表征，结果表明，正确的 Φ(F) 测定受到局部 pH 值的影响，并解决了观察到的辐射增强问题。我们的方法基于实验保证，即参考荧光团与 AuNP-荧光团缀合物具有相同的光学条件。我们证明了引入内滤效应和 AuNPs 引起的发射光再吸收的校正的相关性。所提出的方法可以避免基于理论的校正，并允许更准确地确定 AuNPs 附近的荧光发射。
