Cordeiro M, Giestas L, Lima J C, Baptista P M V
LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516, Caparica, Portugal.
CIGMH, UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516, Caparica, Portugal.
J Nanobiotechnology. 2016 May 17;14(1):38. doi: 10.1186/s12951-016-0192-y.
Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem.
We proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous format whose output capability (i.e. additional layer of information) is potentiated via the spectral codification strategy.
The spectral coded Au-nanobeacons permit the detection of each of the pathogenic fusion sequences, with high specificity towards partial complementary sequences. The proposed BioCode Au-nanobeacon concept provides for a nanoplatform for molecular recognition suitable for cancer diagnostics.
金纳米信标（Au - 纳米信标）已被证明是用于分子诊断和治疗驱动的多功能系统。在此，我们展示了两种结合基于Förster共振能量转移（FRET）的光谱编码用于双模式序列区分的金纳米信标的开发与表征。这是将两种强大技术整合到单个纳米系统中。
我们证明了该概念可检测与慢性髓性白血病发展相关的最常见融合序列，即e13a2和e14a2。检测基于供体信号向受体的光谱位移，这使得能够确证杂交事件。Au - 纳米信标作为以均相形式检测靶标的支架，其输出能力（即额外的信息层）通过光谱编码策略得到增强。
光谱编码的Au - 纳米信标能够检测每种致病融合序列，对部分互补序列具有高度特异性。所提出的生物编码Au - 纳米信标概念为适用于癌症诊断的分子识别提供了一个纳米平台。
