Slamberová Romana, Mikulecká Anna, Pometlová Marie, Schutová Barbora, Hrubá Lenka, Deykun Kateryna
Department of Normal, Pathological, and Clinical Physiology, 3rd Faculty of Medicine, Charles University in Prague, Czech Republic.
Behav Pharmacol. 2011 Oct;22(7):617-23. doi: 10.1097/FBP.0b013e32834afea4.
Our previous study showed that single injection of methamphetamine decreases social interaction (SI) in a dose-dependent manner that was further affected by stressful environment conditions. The aim of this study was to examine the effect of methamphetamine and its interaction with gonadal hormones on SI. Adult male and female rats were gonadectomized and assigned to testosterone-treated and oil-treated groups in male rats and estradiol-treated and oil-treated groups in female rats, respectively. Hormones were administered 30 min before each habituation in the open field. All four hormonal groups were further divided to control (without injection), saline (1 ml/kg saline injection), and methamphetamine (1 mg/kg) groups. Injections were applied 30 min before the SI test. The total duration and the total number of SI and nonsocial behavioral patterns were assessed. This study showed that an acute methamphetamine administration in a dose of 1 mg/kg decreased different types of SI in both sexes. In contrast, the same dose of methamphetamine increased locomotion and rearing behavior in male and female rats. The frequency and/or duration of SI (especially mutual sniffing and allogrooming) was lower in adult female rats relative to gonadectomized male rats, but locomotion was increased in female relative to male rats regardless of the presence or absence of gonadal hormones. In conclusion, this study is novel especially because it examines SI in both sexes in relation to the presence or absence of gonadal hormones.
我们之前的研究表明，单次注射甲基苯丙胺会以剂量依赖的方式减少社交互动（SI），而应激环境条件会进一步影响这种减少。本研究的目的是研究甲基苯丙胺及其与性腺激素的相互作用对社交互动的影响。成年雄性和雌性大鼠接受去势手术，雄性大鼠分别分为睾酮处理组和油处理组，雌性大鼠分别分为雌二醇处理组和油处理组。在每次旷场适应前30分钟给予激素。所有四个激素组进一步分为对照组（不注射）、生理盐水组（注射1 ml/kg生理盐水）和甲基苯丙胺组（1 mg/kg）。在社交互动测试前30分钟进行注射。评估社交互动和非社交行为模式的总时长和总数。本研究表明，急性给予1 mg/kg剂量的甲基苯丙胺会减少两性不同类型的社交互动。相反，相同剂量的甲基苯丙胺会增加雄性和雌性大鼠的运动和竖毛行为。成年雌性大鼠的社交互动频率和/或时长（尤其是相互嗅探和互理被毛）低于去势雄性大鼠，但无论有无性腺激素，雌性大鼠的运动均比雄性大鼠增加。总之，本研究具有创新性，特别是因为它研究了两性在有无性腺激素情况下的社交互动。
