Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh.
BMC Complement Altern Med. 2011 Sep 20;11:76. doi: 10.1186/1472-6882-11-76.
Ginger is one of the most important spice crops and traditionally has been used as medicinal plant in Bangladesh. The present work is aimed to find out antioxidant and anticancer activities of two Bangladeshi ginger varieties (Fulbaria and Syedpuri) at young age grown under ambient (400 μmol/mol) and elevated (800 μmol/mol) CO2 concentrations against two human breast cancer cell lines (MCF-7 and MDA-MB-231).
The effects of ginger on MCF-7 and MDA-MB-231 cell lines were determined using TBA (thiobarbituric acid) and MTT [3-(4,5-dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide] assays. Reversed-phase HPLC was used to assay flavonoids composition among Fulbaria and Syedpuri ginger varieties grown under increasing CO2 concentration from 400 to 800 μmol/mol.
Antioxidant activities in both varieties found increased significantly (P ≤ 0.05) with increasing CO2 concentration from 400 to 800 μmol/mol. High antioxidant activities were observed in the rhizomes of Syedpuri grown under elevated CO2 concentration. The results showed that enriched ginger extract (rhizomes) exhibited the highest anticancer activity on MCF-7 cancer cells with IC50 values of 34.8 and 25.7 μg/ml for Fulbaria and Syedpuri respectively. IC50 values for MDA-MB-231 exhibition were 32.53 and 30.20 μg/ml for rhizomes extract of Fulbaria and Syedpuri accordingly.
Fulbaria and Syedpuri possess antioxidant and anticancer properties especially when grown under elevated CO2 concentration. The use of ginger grown under elevated CO2 concentration may have potential in the treatment and prevention of cancer.
姜是最重要的香料作物之一，在孟加拉国传统上被用作药用植物。本工作旨在寻找两种孟加拉国姜品种（Fulbaria 和 Syedpuri）在年轻时期在环境（400 μmol/mol）和升高（800 μmol/mol）CO2浓度下的抗氧化和抗癌活性，针对两种人乳腺癌细胞系（MCF-7 和 MDA-MB-231）。
使用 TBA（硫代巴比妥酸）和 MTT [3-（4,5-二甲基噻唑基）-2,5-二苯基四唑溴盐]测定姜对 MCF-7 和 MDA-MB-231 细胞系的影响。反相高效液相色谱法用于测定在 CO2 浓度从 400 增加到 800 μmol/mol 时，Fulbaria 和 Syedpuri 姜品种的类黄酮组成。
两种品种的抗氧化活性均随 CO2 浓度从 400 增加到 800 μmol/mol 而显著增加（P≤0.05）。在升高的 CO2 浓度下生长的 Syedpuri 根茎中观察到高抗氧化活性。结果表明，富含姜提取物（根茎）对 MCF-7 癌细胞表现出最高的抗癌活性，IC50 值分别为 Fulbaria 和 Syedpuri 的 34.8 和 25.7μg/ml。MDA-MB-231 表现出的 IC50 值分别为 Fulbaria 和 Syedpuri 根茎提取物的 32.53 和 30.20μg/ml。
Fulbaria 和 Syedpuri 具有抗氧化和抗癌特性，特别是在升高的 CO2 浓度下生长时。使用在升高的 CO2 浓度下生长的姜可能具有在癌症治疗和预防中的潜在应用。
