Department of Medicinal Chemistry, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta 700032, India.
J Ethnopharmacol. 2011 Oct 31;138(1):256-9. doi: 10.1016/j.jep.2011.09.007. Epub 2011 Sep 16.
Commiphora berryi is traditionally used for the treatment of cold and fever as well as for wound healing in the southern parts of India.
The present study was designed to investigate in vitro soybean lipoxygenase inhibitory activity of crude extracts and compounds isolated from Commiphora berryi.
The bark of Commiphora berryi was extracted with different organic solvents and subjected to chromatographic separation for isolation of bioactive compounds. Structures of isolated compounds were elucidated by spectroscopic methods. The anti-inflammatory activity of bark extracts and bioactive compounds were assessed by in vitro soybean lipoxygenase (SBL) assay.
3β-Hydroxyglutin-5-ene (1), friedelin (2), cycloeucaneol (3) nimbiol (4), sugiol (5), surianol (6), daucosterol (7) and ursolic acid (8) were isolated from crude bark extracts of the Commiphora berryi. The structure of nimbiol (4) was also confirmed by single crystal X-ray analysis. The petroleum ether, methanol, chloroform and ethyl acetate extracts of bark of Commiphora berryi showed SBL inhibitory activity with the IC(50) values of 15.3, 54.2, 71.5 and 87.8 μg/ml respectively. Among all the isolates, friedelin (2) showed significant SBL inhibitory activity with IC(50) 35.8 μM.
The overall results provide evidence that the studied plant might be a potential source of anti-inflammatory agents.
在印度南部， Commiphora berryi 传统上被用于治疗感冒和发烧以及伤口愈合。
本研究旨在研究 Commiphora berryi 的粗提取物和分离化合物对体外大豆脂氧合酶的抑制活性。
用不同的有机溶剂从 Commiphora berryi 的树皮中提取，并进行色谱分离以分离生物活性化合物。通过光谱方法阐明分离化合物的结构。通过体外大豆脂氧合酶（SBL）测定评估树皮提取物和生物活性化合物的抗炎活性。
从 Commiphora berryi 的粗树皮提取物中分离出 3β-羟基-5-烯（1）、friedelin（2）、cycloeucaneol（3）、nimbiol（4）、sugiol（5）、surianol（6）、daucosterol（7）和ursolic acid（8）。nimbiol（4）的结构也通过单晶 X 射线分析得到证实。 Commiphora berryi 树皮的石油醚、甲醇、氯仿和乙酸乙酯提取物均表现出 SBL 抑制活性，IC（50）值分别为 15.3、54.2、71.5 和 87.8μg/ml。在所有分离物中，friedelin（2）表现出显著的 SBL 抑制活性，IC（50）为 35.8μM。
总体结果表明，研究植物可能是抗炎剂的潜在来源。
