miRNA 筛选揭示了一个新的 miRNA 家族，通过调节 Meox2 来刺激 iPS 细胞的生成。
miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2.
机构信息
REBIRTH-Group Reprogramming, Hannover Medical School, 30625 Hannover, Germany.
出版信息
EMBO Rep. 2011 Oct 28;12(11):1153-9. doi: 10.1038/embor.2011.176.
Abstract
Induced pluripotent stem cells (iPSCs) can be generated by overexpression of Oct4, Sox2 and Klf4 in murine fibroblasts. By conducting a microRNA (miRNA) library screen, we identified a set of miRNAs critically regulating iPSC formation. We revealed a new miRNA family (miR-130/301/721) as an important regulator of iPSC induction by targeting the homeobox transcription factor Meox2 (also known as Gax). Meox2-specific silencing mimicked the effects of this miRNA family on reprogramming. Mechanistically, miRNA-resistant Meox2 overexpression abrogated effects of miR-130/301/721 on reprogramming. In conclusion, the miRNA family miR-130/301/721 enhances iPSC generation via repression of Meox2.
摘要
诱导多能干细胞（iPSCs）可通过在鼠成纤维细胞中过表达 Oct4、Sox2 和 Klf4 来生成。通过进行 microRNA（miRNA）文库筛选，我们鉴定出一组 miRNA 对 iPSC 形成具有关键调控作用。我们揭示了一个新的 miRNA 家族（miR-130/301/721）作为 iPSC 诱导的重要调节剂，通过靶向同源盒转录因子 Meox2（也称为 Gax）发挥作用。Meox2 特异性沉默模拟了该 miRNA 家族对重编程的作用。从机制上讲，miRNA 抗性 Meox2 过表达消除了 miR-130/301/721 对重编程的影响。总之，miRNA 家族 miR-130/301/721 通过抑制 Meox2 增强 iPSC 的生成。
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