单细胞水平的基因组分析。
Genomic analysis at the single-cell level.
机构信息
Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, Stanford, California 94305, USA.
出版信息
Annu Rev Genet. 2011;45:431-45. doi: 10.1146/annurev-genet-102209-163607. Epub 2011 Sep 19.
Abstract
Studying complex biological systems such as a developing embryo, a tumor, or a microbial ecosystem often involves understanding the behavior and heterogeneity of the individual cells that constitute the system and their interactions. In this review, we discuss a variety of approaches to single-cell genomic analysis.
摘要
研究复杂的生物系统，如发育中的胚胎、肿瘤或微生物生态系统，通常需要了解构成系统的单个细胞的行为和异质性及其相互作用。在这篇综述中，我们讨论了多种单细胞基因组分析方法。
相似文献
1
Genomic analysis at the single-cell level.单细胞水平的基因组分析。
Annu Rev Genet. 2011;45:431-45. doi: 10.1146/annurev-genet-102209-163607. Epub 2011 Sep 19.
2
Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis.实现高通量单细胞分析的微滴微流控技术的发展
Molecules. 2016 Jul 5;21(7):881. doi: 10.3390/molecules21070881.
3
Chromium 10× Single-Cell 3' mRNA Sequencing of Tumor-Infiltrating Lymphocytes.肿瘤浸润淋巴细胞的铬10×单细胞3' mRNA测序
Methods Mol Biol. 2019;1979:87-110. doi: 10.1007/978-1-4939-9240-9_7.
4
Single cell screening approaches for antibody discovery.用于抗体发现的单细胞筛选方法。
Methods. 2017 Mar 1;116:34-42. doi: 10.1016/j.ymeth.2016.11.006. Epub 2016 Nov 15.
5
Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics.单细胞高通量技术在脑脊液研究和诊断中的应用。
Front Immunol. 2019 Jun 11;10:1302. doi: 10.3389/fimmu.2019.01302. eCollection 2019.
6
High Throughput Multi-Omics Approaches for Clinical Trial Evaluation and Drug Discovery.用于临床试验评估和药物发现的高通量多组学方法
Front Immunol. 2021 Mar 31;12:590742. doi: 10.3389/fimmu.2021.590742. eCollection 2021.
7
Improvements in high-throughput, high-content analysis of single cells.单细胞高通量、高内涵分析的进展。
Cytometry A. 2013 Apr;83(4):331-2. doi: 10.1002/cyto.a.22285.
8
Surface-printed microdot array chips coupled with matrix-assisted laser desorption/ionization mass spectrometry for high-throughput single-cell patterning and phospholipid analysis.表面印刷微点阵列芯片与基质辅助激光解吸/电离质谱联用，用于高通量单细胞图案化和磷脂分析。
Rapid Commun Mass Spectrom. 2016 Aug;30 Suppl 1:73-9. doi: 10.1002/rcm.7628.
9
High-throughput, multiparameter analysis of single cells.单细胞的高通量多参数分析。
Anal Bioanal Chem. 2014 May;406(14):3279-96. doi: 10.1007/s00216-013-7485-x. Epub 2013 Dec 1.
10
Integrated, multi-scale, spatial-temporal cell biology--A next step in the post genomic era.整合的、多尺度的时空细胞生物学——后基因组时代的下一步。
Methods. 2016 Mar 1;96:3-5. doi: 10.1016/j.ymeth.2015.09.007. Epub 2015 Sep 7.
引用本文的文献
1
IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation.胰岛素样生长因子结合蛋白3通过激活整合素β1和细胞外信号调节激酶通路增强脂肪来源干细胞在软组织损伤修复中的功能。
Cell Biol Toxicol. 2025 May 15;41(1):85. doi: 10.1007/s10565-025-10024-8.
2
Advances in Microfluidic Single-Cell RNA Sequencing and Spatial Transcriptomics.微流控单细胞RNA测序与空间转录组学的进展
Micromachines (Basel). 2025 Apr 2;16(4):426. doi: 10.3390/mi16040426.
3
Integrative analysis of ferroptosis in the hypoxic microenvironment of gastric cancer unveils the immune landscape and personalized therapeutic strategies.胃癌缺氧微环境中细胞铁死亡的综合分析揭示了免疫格局和个性化治疗策略。
Front Oncol. 2025 Jan 13;14:1499580. doi: 10.3389/fonc.2024.1499580. eCollection 2024.
4
Single cell transcriptomics in blood of patients with chronic obstructive pulmonary disease.慢性阻塞性肺疾病患者血液中的单细胞转录组学
BMC Pulm Med. 2025 Jan 14;25(1):19. doi: 10.1186/s12890-024-03475-y.
5
A novel spherical GelMA-HAMA hydrogel encapsulating APET×2 polypeptide and CFIm25-targeting sgRNA for immune microenvironment modulation and nucleus pulposus regeneration in intervertebral discs.一种新型的球形 GelMA-HAMA 水凝胶，包封 APET×2 多肽和靶向 CFIm25 的 sgRNA，用于调节椎间盘免疫微环境和促进椎间盘核再生。
J Nanobiotechnology. 2024 Sep 12;22(1):556. doi: 10.1186/s12951-024-02783-z.
6
A new and effective two-step clustering approach for single cell RNA sequencing data.一种新的、有效的单细胞 RNA 测序数据两步聚类方法。
BMC Genomics. 2023 Nov 9;23(Suppl 6):864. doi: 10.1186/s12864-023-09577-x.
7
Improved single-cell genome amplification by a high-efficiency phi29 DNA polymerase.通过高效 phi29 DNA 聚合酶改进单细胞基因组扩增。
Front Bioeng Biotechnol. 2023 Jun 29;11:1233856. doi: 10.3389/fbioe.2023.1233856. eCollection 2023.
8
Bioinformatics and pathway enrichment analysis identified hub genes and potential biomarker for gastric cancer prognosis.生物信息学和通路富集分析确定了胃癌预后的枢纽基因和潜在生物标志物。
Front Oncol. 2023 Jun 9;13:1187521. doi: 10.3389/fonc.2023.1187521. eCollection 2023.
9
Light-driven peristaltic pumping by an actuating splay-bend strip.光驱动的曲折条致动蠕动泵。
Nat Commun. 2023 Apr 4;14(1):1877. doi: 10.1038/s41467-023-37445-5.
10
Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing.结合扩张卷积神经网络和无标记声传感的自动细胞类型分类。
Sci Rep. 2022 Nov 18;12(1):19873. doi: 10.1038/s41598-022-22075-6.
本文引用的文献
1
High-throughput microfluidic single-cell RT-qPCR.高通量微流控单细胞 RT-qPCR。
Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):13999-4004. doi: 10.1073/pnas.1019446108. Epub 2011 Aug 1.
2
Origins of the E. coli strain causing an outbreak of hemolytic-uremic syndrome in Germany.导致德国爆发溶血性尿毒综合征的大肠杆菌菌株的起源。
N Engl J Med. 2011 Aug 25;365(8):709-17. doi: 10.1056/NEJMoa1106920. Epub 2011 Jul 27.
3
Counting individual DNA molecules by the stochastic attachment of diverse labels.通过随机附着不同标记来计数单个 DNA 分子。
Proc Natl Acad Sci U S A. 2011 May 31;108(22):9026-31. doi: 10.1073/pnas.1017621108. Epub 2011 May 11.
4
Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.单细胞质谱流式细胞术分析人类造血连续统中的免疫和药物反应差异。
Science. 2011 May 6;332(6030):687-96. doi: 10.1126/science.1198704.
5
Immunology. Flow cytometry, amped up.免疫学。流式细胞术，升级强化。
Science. 2011 May 6;332(6030):677-8. doi: 10.1126/science.1206351.
6
Development and applications of single-cell transcriptome analysis.单细胞转录组分析的发展与应用。
Nat Methods. 2011 Apr;8(4 Suppl):S6-11. doi: 10.1038/nmeth.1557.
7
Single-cell gene-expression profiling reveals qualitatively distinct CD8 T cells elicited by different gene-based vaccines.单细胞基因表达谱分析揭示了不同基于基因的疫苗引发的定性上不同的 CD8 T 细胞。
Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5724-9. doi: 10.1073/pnas.1013084108. Epub 2011 Mar 21.
8
Tumour evolution inferred by single-cell sequencing.单细胞测序推断肿瘤进化。
Nature. 2011 Apr 7;472(7341):90-4. doi: 10.1038/nature09807. Epub 2011 Mar 13.
9
Caenorhabditis elegans aristaless/Arx gene alr-1 restricts variable gene expression.秀丽隐杆线虫aristaless/Arx 基因 alr-1 限制可变基因表达。
Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4063-8. doi: 10.1073/pnas.1101329108. Epub 2011 Feb 22.
10
Genome of a low-salinity ammonia-oxidizing archaeon determined by single-cell and metagenomic analysis.单细胞和宏基因组分析确定的耐低盐氨氧化古菌基因组。
PLoS One. 2011 Feb 22;6(2):e16626. doi: 10.1371/journal.pone.0016626.
Zotero 插件