Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK.
Nat Methods. 2011 Apr;8(4 Suppl):S6-11. doi: 10.1038/nmeth.1557.
Dissecting the relationship between genotype and phenotype is one of the central goals in developmental biology and medicine. Transcriptome analysis is a powerful strategy to connect genotype to phenotype of a cell. Here we review the history, progress, potential applications and future developments of single-cell transcriptome analysis. In combination with live cell imaging and lineage tracing, it will be possible to decipher the full gene expression network underlying physiological functions of individual cells in embryos and adults, and to study diseases.
解析基因型与表型之间的关系是发育生物学和医学的核心目标之一。转录组分析是将基因型与细胞表型联系起来的一种强大策略。本文综述了单细胞转录组分析的历史、进展、潜在应用和未来发展。与活细胞成像和谱系追踪相结合，将有可能破译胚胎和成年个体细胞中生理功能背后的完整基因表达网络，并研究疾病。
