Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, 28040 Madrid, Spain.
Infect Genet Evol. 2012 Jun;12(4):866-72. doi: 10.1016/j.meegid.2011.09.004. Epub 2011 Sep 14.
Mycobacterium bovis isolates from the Iberian Peninsula are dominated by strains with spoligotype patterns deleted for spacer 21. Whole-genome sequencing of three Spanish strains with spacer 21 missing in their spoligotype pattern revealed a series of SNPs and subsequent screening of a selection of these SNPs identified one in gene guaA that is specific to these strains. This group of strains from the Iberian Peninsula missing spoligotype spacer 21 represents a new clonal complex of M. bovis, defined by the SNP profile with a distinct spoligotype signature. We have named this clonal complex European 2 (Eu2) and found that it was present at low frequency in both France and Italy and absent from the British Isles.
来自伊比利亚半岛的牛分枝杆菌分离株主要是缺失 spoligotype 21 间隔子的菌株。对西班牙的 3 株缺失 spoligotype 21 间隔子的菌株进行全基因组测序，发现了一系列 SNP，并对这些 SNP 进行了筛选，确定了一个位于基因 guaA 的 SNP，该 SNP 是这些菌株所特有的。来自伊比利亚半岛的缺失 spoligotype 21 间隔子的这组菌株代表了一种新的牛分枝杆菌克隆复合体，其 SNP 谱具有独特的 spoligotype 特征。我们将这个克隆复合体命名为欧洲 2 型（Eu2），并发现它在法国和意大利的频率较低，在不列颠群岛则不存在。
