Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Melle, Belgium.
Prev Vet Med. 2012 Mar 1;103(4):298-303. doi: 10.1016/j.prevetmed.2011.09.007. Epub 2011 Sep 25.
This study was part of a larger study that addressed the effects of marine algae (ALG) supplementation in the ration of high yielding periparturient dairy cows. The objectives were to induce milk fat depression (MFD) in early lactation by feeding docosahexaenoic acid (DHA) from ALG and to determine the effects on milk production, milk components and metabolic status early post partum. This study focuses on the oxidative status in the plasma during the ALG supplementation. Plasma samples were collected from 16 Holstein Friesian cows at the day of parturition and at -1, 2, 4 and 6 weeks relative to calving with half of the cows receiving the ALG supplement (44gDHA/d) from 3 weeks pre partum on. The following parameters were measured in plasma: ferric reducing ability of plasma (FRAP), α-tocopherol level, glutathione peroxidase activity (GSH-Px) and thiobarbituric acid reactive substances (TBARS) concentration. There was a significant effect of time for FRAP and α-tocopherol indicating changes in the plasma oxidative status around parturition. The ALG supplementation was successful in creating a milk fat depression (MFD) but could not improve the energy balance. Feeding of ALG significantly increased lipid peroxidation as measured by TBARS, probably through their high content of n-3 polyunsaturated fatty acids.
本研究是一项更大规模研究的一部分，该研究旨在探讨海藻（ALG）补充剂对高产围产期奶牛日粮的影响。目的是通过饲喂海藻来源的二十二碳六烯酸（DHA）来诱导泌乳早期乳脂下降（MFD），并确定其对产后早期产奶量、乳成分和代谢状况的影响。本研究重点关注 ALG 补充期间血浆中的氧化状态。在分娩当天和产后 -1、2、4 和 6 周，从 16 头荷斯坦弗里生奶牛中采集血浆样本，其中一半奶牛从产前 3 周开始接受 ALG 补充剂（44gDHA/d）。在血浆中测量了以下参数：血浆铁还原能力（FRAP）、α-生育酚水平、谷胱甘肽过氧化物酶活性（GSH-Px）和硫代巴比妥酸反应物质（TBARS）浓度。FRAP 和 α-生育酚的时间效应显著，表明围产期前后血浆氧化状态发生变化。ALG 补充成功地造成了乳脂下降（MFD），但不能改善能量平衡。通过 TBARS 测量，饲喂 ALG 显著增加了脂质过氧化，这可能是由于其富含 n-3 多不饱和脂肪酸。
