Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.
Biosens Bioelectron. 2011 Dec 15;30(1):67-72. doi: 10.1016/j.bios.2011.08.028. Epub 2011 Aug 27.
A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxyfluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic Escherichia coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin) and lipases (phospholipase A(2)). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S. aureus (MSSA 476) and P. aeruginosa (PAO1) but not E. coli DH5α in a prototype dressing.
已经开发出一种新的方法来检测伤口敷料的微生物状态，即检测金黄色葡萄球菌或铜绿假单胞菌等致病细菌的定植情况。在这里，我们报告了如何使含有自猝灭羧基荧光素染料的稳定脂质体仅对铜绿假单胞菌和金黄色葡萄球菌的毒素/毒力因子敏感，而对非毒性的大肠杆菌物种不敏感。讨论了稳定脂质体的发展，并研究了它们对去污剂（吐温）、细菌毒素（α-溶血素）和脂肪酶（磷脂酶 A(2)）的反应。最后，通过等离子体沉积马来酸酐偶联剂将稳定的脂质体附着在织物上，通过原型敷料可以明显地观察到对金黄色葡萄球菌（MSSA476）和铜绿假单胞菌（PAO1）的响应，但对大肠杆菌 DH5α 没有响应。
