Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
J Agric Food Chem. 2011 Nov 23;59(22):12193-7. doi: 10.1021/jf202837g. Epub 2011 Oct 31.
A peptide possessing antihypertensive activity was purified from Nile tilapia ( Oreochromis niloticus ) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods.
一种具有降血压活性的肽从尼罗罗非鱼（Oreochromis niloticus）明胶中用碱性蛋白酶、胰蛋白酶、胃蛋白酶和蛋白酶 E 进行分离和纯化。其中，碱性蛋白酶水解物具有最高的血管紧张素转化酶（ACE）抑制活性。因此，对其进行了进一步分析，并分离和纯化了一种具有强效 ACE 抑制活性的肽 DPALATEPDPMPF（1382 Da）。此外，还测定了该纯化肽对小鼠小胶质细胞（BV-2）中自由基诱导的细胞和 DNA 损伤的保护作用。这些结果表明，从尼罗罗非鱼（O. niloticus）明胶中分离出的肽可作为一种抗高血压和抗氧化应激的候选物，并可用于功能性食品。
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