Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Inflammopharmacology. 2011 Dec;19(6):307-16. doi: 10.1007/s10787-011-0092-6. Epub 2011 Sep 23.
The present study was designed to examine the potential preventive and curative effects of curcumin, resveratrol, imatinib, rosiglitazone, losartan and bosentan (BOS) on Schistosoma mansoni-induced liver fibrosis in mice. Induction of liver fibrosis was produced in male Swiss mice by subcutaneous injection of S. mansoni cercariae per mouse. Mice were left for 28 days before starting the experiment then mice were divided into two main groups. The first group was further subdivided into experimental groups and started drug treatment at day 28 after infection and continued for 2 weeks in order to evaluate the potential preventive effects of the mentioned drugs on S. mansoni-induced liver fibrosis. The second group of mice were left for 2 weeks and then treated with praziquantel for two consecutive days to eradicate the worms and so stop egg disposition and further fibrosis development. Mice were then subdivided into the experimental groups and drug treatment was started for 2 weeks to evaluate their efficacy to decrease the developed fibrosis. At the end of the experiment period, mice were killed and serum was collected for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin and albumin. Liver tissue was taken for the estimation of hepatic hydroxyproline content and histopathological examination to confirm the biochemical results. Results of the study indicate that curcumin and imatinib have potent antifibrotic activity both in suppressing and reversing S. mansoni-induced liver fibrosis, while resveratrol has beneficial effects only in suppressing the development of S. mansoni-induced liver fibrosis.
本研究旨在探讨姜黄素、白藜芦醇、伊马替尼、罗格列酮、氯沙坦和波生坦（BOS）对曼氏血吸虫诱导的肝纤维化的潜在预防和治疗作用。通过皮下注射曼氏血吸虫尾蚴，在雄性瑞士小鼠中诱导肝纤维化。在开始实验前，将小鼠饲养 28 天，然后将小鼠分为两组。第一组进一步分为实验组，在感染后第 28 天开始药物治疗，并持续 2 周，以评估上述药物对曼氏血吸虫诱导的肝纤维化的潜在预防作用。第二组小鼠饲养 2 周，然后连续 2 天用吡喹酮治疗以消灭蠕虫，从而阻止虫卵的排放和进一步的纤维化发展。然后将小鼠分为实验组，并开始药物治疗 2 周，以评估其减少已发展纤维化的疗效。实验结束时，处死小鼠并收集血清，用于测定丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、胆红素和白蛋白。取肝组织用于测定肝羟脯氨酸含量，并进行组织病理学检查以确认生化结果。研究结果表明，姜黄素和伊马替尼均具有抑制和逆转曼氏血吸虫诱导的肝纤维化的强大抗纤维化活性，而白藜芦醇仅具有抑制曼氏血吸虫诱导的肝纤维化发展的有益作用。
