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Comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of Mycobacterium paratuberculosis in goats.

作者信息

Thoresen O F, Falk K, Evensen O

机构信息

Central Veterinary Laboratory, Oslo, Norway.

出版信息

J Vet Diagn Invest. 1994 Apr;6(2):195-9. doi: 10.1177/104063879400600210.

DOI:10.1177/104063879400600210
PMID:8068751
Abstract

Forty-seven paired specimens of ileum and mesenterial lymph nodes from goats originating from 2 herds with paratuberculosis were investigated. Culture of the specimens for Mycobacterium paratuberculosis was compared with Ziehl-Neelsen staining and with immunohistochemical tests on paraffin-embedded tissue sections, using a M. paratuberculosis immune serum and an avidin-biotin-alkaline phosphatase method. Immunohistochemical techniques detected most positive samples and produced more clearly visible reactions than did acid-fast staining. Eighteen of 47 samples were positive by immunohistochemical techniques may represent a valuable adjunct to standard techniques for diagnosis of paratuberculosis in goats.

摘要

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