The University of Western Ontario - Department of Biochemistry and Schulich Dentistry, Schulich School of Medicine and Dentistry, London, ON, Canada.
Proteomics Clin Appl. 2011 Dec;5(11-12):575-9. doi: 10.1002/prca.201100046.
We provide a brief overview of the salivary proteome but with an emphasis on the major challenges in protein identification and quantitation. Precautions are necessary to avoid proteolysis, deglycosylation and dephosphorylation of salivary proteins by microbial and host enzymes in saliva. Many proteins are differentially expressed in secretions from different salivary glands and their proportional contributions to saliva vary with the flow rate. The total protein concentration in the secretion from any one gland varies considerably, depending on factors such as flow rate, duration of stimulation, nature of the stimulus and circadian rhythms. Many plasma proteins enter saliva via gingival crevicular fluid, of which there are increased amounts in persons with gingivitis or periodontal disease. These factors must be taken into account in the identification of potential biomarkers for different oral or systemic diseases.
我们简要概述了唾液蛋白质组学，但重点介绍了蛋白质鉴定和定量方面的主要挑战。需要注意的是，要避免唾液中的微生物和宿主酶对唾液蛋白质的蛋白水解、去糖基化和去磷酸化。许多蛋白质在不同唾液腺分泌的分泌物中表达不同，它们对唾液的比例随流速而变化。任何一个腺体分泌液中的总蛋白浓度变化很大，这取决于流速、刺激持续时间、刺激性质和昼夜节律等因素。许多血浆蛋白通过牙龈沟液进入唾液，患有牙龈炎或牙周病的人其牙龈沟液中的含量会增加。在鉴定不同口腔或系统性疾病的潜在生物标志物时，必须考虑这些因素。
