Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, Osaka, Japan.
Magn Reson Med Sci. 2011;10(3):169-76. doi: 10.2463/mrms.10.169.
We assessed intrarenal oxygenation in gentamicin-induced nephrotoxicity (GIN) and the protective effect of hydrogen-rich water (HW) against GIN using blood oxygenation level-dependent magnetic resonance (MR) imaging.
We acquired T(2)-weighted images (T(2)WI) of 21 rats on Days 0, 2, 4, and 7 using a 1.5-tesla MR imaging system. The rats were divided into 3 groups of seven each: control rats had free access to standard water and no gentamicin (GM) injection; rats designated the GM group had free access to standard water and were injected with GM (80 mg/kg/day) subcutaneously for 7 days; and the third group, designated the GM+HW group, had free access to HW and were injected with GM. R(2) (=1/T(2)) was estimated from T(2)*WI.
R(2)* values in the cortex were significantly decreased on Days 2, 4, and 7 compared with those on Day 0 in the GM group but not significantly changed in the control and GM+HW groups. R(2)* values in the medulla did not change significantly in any group.
Our findings suggested reduced oxygen utility, mainly in the cortex, in gentamicin-induced nephrotoxicity and an ameliorative effect of hydrogen-rich water against GIN.
本研究采用血氧水平依赖磁共振成像（BOLD-MRI）评估庆大霉素诱导的肾毒性（GIN）中的肾内氧合，并评估富氢水（HW）对 GIN 的保护作用。
使用 1.5T 磁共振成像系统对 21 只大鼠在第 0、2、4 和 7 天进行 T2加权图像（T2WI）采集。大鼠分为三组，每组 7 只：对照组大鼠可自由饮用标准水且不注射庆大霉素（GM）；GM 组大鼠可自由饮用标准水且连续 7 天每天皮下注射 GM（80mg/kg）；第三组为 GM+HW 组，可自由饮用 HW 且注射 GM。从 T2WI 中估算 R（2）（=1/T2*）。
与第 0 天相比，GM 组大鼠的皮质 R（2）*值在第 2、4 和 7 天显著降低，但对照组和 GM+HW 组大鼠的 R（2）*值无显著变化。各组大鼠的髓质 R（2）*值均无明显变化。
我们的研究结果提示庆大霉素诱导的肾毒性可导致氧利用减少，主要发生在皮质，而富氢水具有改善 GIN 的作用。
