利用血氧水平依赖磁共振成像研究富氢水对大鼠庆大霉素肾毒性的保护作用。

Protective effect of hydrogen-rich water against gentamicin-induced nephrotoxicity in rats using blood oxygenation level-dependent MR imaging.

机构信息

Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, Osaka, Japan.

出版信息

Magn Reson Med Sci. 2011;10(3):169-76. doi: 10.2463/mrms.10.169.

DOI:10.2463/mrms.10.169

PMID:21959999

Abstract

PURPOSE

We assessed intrarenal oxygenation in gentamicin-induced nephrotoxicity (GIN) and the protective effect of hydrogen-rich water (HW) against GIN using blood oxygenation level-dependent magnetic resonance (MR) imaging.

MATERIALS AND METHODS

We acquired T(2)-weighted images (T(2)WI) of 21 rats on Days 0, 2, 4, and 7 using a 1.5-tesla MR imaging system. The rats were divided into 3 groups of seven each: control rats had free access to standard water and no gentamicin (GM) injection; rats designated the GM group had free access to standard water and were injected with GM (80 mg/kg/day) subcutaneously for 7 days; and the third group, designated the GM+HW group, had free access to HW and were injected with GM. R(2) (=1/T(2)) was estimated from T(2)*WI.

RESULTS

R(2)* values in the cortex were significantly decreased on Days 2, 4, and 7 compared with those on Day 0 in the GM group but not significantly changed in the control and GM+HW groups. R(2)* values in the medulla did not change significantly in any group.

CONCLUSIONS

Our findings suggested reduced oxygen utility, mainly in the cortex, in gentamicin-induced nephrotoxicity and an ameliorative effect of hydrogen-rich water against GIN.

摘要

目的

本研究采用血氧水平依赖磁共振成像（BOLD-MRI）评估庆大霉素诱导的肾毒性（GIN）中的肾内氧合，并评估富氢水（HW）对 GIN 的保护作用。

材料与方法

使用 1.5T 磁共振成像系统对 21 只大鼠在第 0、2、4 和 7 天进行 T2加权图像（T2WI）采集。大鼠分为三组，每组 7 只：对照组大鼠可自由饮用标准水且不注射庆大霉素（GM）；GM 组大鼠可自由饮用标准水且连续 7 天每天皮下注射 GM（80mg/kg）；第三组为 GM+HW 组，可自由饮用 HW 且注射 GM。从 T2WI 中估算 R（2）（=1/T2*）。