Saska S, Barud H S, Gaspar A M M, Marchetto R, Ribeiro S J L, Messaddeq Y
Institute of Chemistry, University Estadual Paulista-UNESP, CP 355, 14-801-970 Araraquara, SP, Brazil.
Int J Biomater. 2011;2011:175362. doi: 10.1155/2011/175362. Epub 2011 Sep 27.
The aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl(2) followed by Na(2)HPO(4). BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40%-50% of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration.
本研究的目的是开发并评估用于骨再生的细菌纤维素 - 羟基磷灰石（BC - HA）纳米复合膜的生物学特性。纳米复合材料由细菌纤维素膜依次在氯化钙溶液和磷酸氢二钠溶液中孵育制备而成。在大鼠胫骨的非关键性骨缺损处，于1周、4周和16周时对BC - HA膜进行评估。热重分析表明，矿物相的量占总重量的40% - 50%。光谱学、电子显微镜/能量色散X射线分析以及X射线衍射表明在BC纳米纤维上形成了HA晶体。低结晶度的HA晶体呈现出1.5的钙磷摩尔比（缺钙型HA），与生理骨相似。傅里叶变换红外光谱分析显示出归属于磷酸根和碳酸根离子的谱带。体内试验表明1周后无炎症反应。4周后，观察到缺损被新骨组织完全填充。BC - HA膜对骨再生有效。
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