KUYTAM Surface Science and Technology Center, Chemistry Department, KOÇ University, Istanbul, Turkey.
KUYTAM Surface Science and Technology Center, Chemistry Department, KOÇ University, Istanbul, Turkey.
Carbohydr Polym. 2017 Nov 1;175:38-46. doi: 10.1016/j.carbpol.2017.07.054. Epub 2017 Jul 22.
Preparation and characterization of chitosan/hydroxyapatite (CS/HA) nanocomposites displaying an intercalated structure is reported. Hydroxyapatite was synthesized through sol-gel process. Formic acid was introduced as a new solvent to obtain stable dispersions of nano-sized HA particles in polymer solution. CS/HA dispersions with HA contents of 5, 10 and 20% by weight were prepared. Self-assembling of HA nanoparticles during the drying of the solvent cast films led to the formation of homogeneous CS/HA nanocomposites. Composite films were analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive X-rays (EDX) analysis, Fourier transform infrared (FTIR) spectroscopy, X-rays diffraction (XRD) analysis and thermogravimetric analysis (TGA). SEM and AFM confirmed the presence of uniformly distributed HA nanoparticles on the chitosan matrix surface. XRD patterns and cross-sectional SEM images showed the formation of layered nanocomposites. Complete degradation of chitosan matrix in TGA experiments, led to the formation of nanoporous 3D scaffolds containing hydroxyapatite, β-tricalcium phosphate and calcium pyrophosphate. CS/HA composites can be considered as promising materials for bone tissue engineering applications.
报道了一种具有插层结构的壳聚糖/羟基磷灰石（CS/HA）纳米复合材料的制备与表征。通过溶胶-凝胶法合成了羟基磷灰石。引入甲酸作为新溶剂，以获得聚合物溶液中纳米级 HA 颗粒的稳定分散体。制备了 HA 含量分别为 5wt%、10wt%和 20wt%的 CS/HA 分散体。溶剂浇铸膜干燥过程中 HA 纳米颗粒的自组装导致了均匀的 CS/HA 纳米复合材料的形成。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）、能谱分析（EDX）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和热重分析（TGA）对复合膜进行了分析。SEM 和 AFM 证实了均匀分布的 HA 纳米颗粒存在于壳聚糖基质表面。XRD 图谱和截面 SEM 图像显示了层状纳米复合材料的形成。TGA 实验中壳聚糖基质的完全降解导致了含有羟基磷灰石、β-磷酸三钙和焦磷酸钙的纳米多孔 3D 支架的形成。CS/HA 复合材料可被视为用于骨组织工程应用的有前途的材料。
