Khodadad Nastaran, Hashempour Ava, Akbarinia Shokufeh
HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Sci Rep. 2025 Jan 2;15(1):326. doi: 10.1038/s41598-024-84376-2.
Investigations of the K1 gene revealed six main genotypes clustered according to geography. Here, the global distribution and HHV8 genotyping using the K1 gene and two hypervariable regions (VR1 and VR2) were evaluated. We searched GenBank for 6,889 HHV8-K1 genes via various keywords, selecting sequences longer than 730 bp. Afterwards, the VR1 and VR2 regions were derived from the K1 genes, and genotyping of the K1, VR1, and VR2 sequences was performed by applying phylogenetic tree and BioAfrica methods. The K1 genotyping result was most similar to that of VR1, followed by VR2. The most common genotypes and subtypes in the three regions studied were A (A2) and C (C3), which are found in Africa, America, and Asia. Although the A and C genotypes are more predominant, the other genotypes, B, D, E, and F, are more ancient and are commonly found in America, Asia, and Oceania. K1 is commonly used for HHV8 genotyping, but VR1 can be a reliable alternative when long-term PCR amplification is not possible. The genotyping and subtyping results of both methods were very similar (92%), and it can be inferred that both procedures can be applied for HHV-8 genotyping.
对K1基因的研究揭示了根据地理位置聚类的六种主要基因型。在此，我们评估了使用K1基因和两个高变区（VR1和VR2）的HHV8全球分布及基因分型情况。我们通过各种关键词在GenBank中搜索了6889个HHV8 - K1基因，选择长度超过730 bp的序列。之后，从K1基因中获取VR1和VR2区域，并通过应用系统发育树和BioAfrica方法对K1、VR1和VR2序列进行基因分型。K1基因分型结果与VR1的最为相似，其次是VR2。在所研究的三个区域中，最常见的基因型和亚型是A（A2）和C（C3），在非洲、美洲和亚洲均有发现。尽管A和C基因型更为 predominant，但其他基因型B、D、E和F更为古老，常见于美洲、亚洲和大洋洲。K1通常用于HHV8基因分型，但当无法进行长期PCR扩增时，VR1可以是一种可靠的替代方法。两种方法的基因分型和亚型分型结果非常相似（92%），可以推断这两种方法均可用于HHV - 8基因分型。 （注：原文中“predominant”拼写错误，正确拼写为“predominant”，翻译为“占主导地位的” ）
