Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, 14155, Iran.
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
Virol J. 2021 Jul 10;18(1):144. doi: 10.1186/s12985-021-01614-z.
Regard to this fact that the main transmission route of HPV and HHV-8 is via sexual activity, it is reasonable to speculate that coinfection of HPV and HHV-8 may have been played an important role in the development of cervical cancer. The aim of this study was to estimate the prevalence of HHV-8 and the frequency of HPV and HHV-8 coinfection in cervical samples of patients with cervical cancer and healthy individuals.
In total, 364 samples from 61 patients with cervical cancer, 124 women with premalignant lesions, and 179 healthy individuals were investigated by nested-PCR.
The frequency of HHV-8 was found to be 22.9%, 17.7%, and 14.5% in cervical cancer, premalignant lesions, and normal specimens, respectively (P = 0.308). The overall prevalence of coinfection between HHV-8 and HPV was shown to be 16.2%. The HPV prevalence was higher in HHV-8 positive samples than HHV-8 negative specimens in all three studied groups and this difference was reached a statistically significant level (P = 0.002). However, no significant differences were found between HHV-8 positivity and HPV genotypes (P = 0.08).
Our results showed the higher rate of HHV-8 genome detection in cervical cancer group than control group. However, future studies with larger sample sizes and evaluation of expression of HHV-8 proteins are warranted.
鉴于 HPV 和 HHV-8 的主要传播途径是通过性行为，因此可以推测 HPV 和 HHV-8 的合并感染可能在宫颈癌的发展中起了重要作用。本研究的目的是评估人疱疹病毒 8 （HHV-8）在宫颈癌患者和健康个体宫颈样本中的流行率以及 HPV 和 HHV-8 合并感染的频率。
总共对 61 例宫颈癌患者、124 例癌前病变患者和 179 例健康个体的 364 个样本进行了巢式-PCR 检测。
宫颈癌、癌前病变和正常标本中 HHV-8 的频率分别为 22.9%、17.7%和 14.5%（P = 0.308）。HHV-8 和 HPV 之间合并感染的总流行率为 16.2%。在所有三组研究中，HHV-8 阳性样本中的 HPV 流行率均高于 HHV-8 阴性样本，且差异具有统计学意义（P = 0.002）。然而，在 HHV-8 阳性和 HPV 基因型之间未发现显著差异（P = 0.08）。
我们的结果表明，宫颈癌组中 HHV-8 基因组的检测率高于对照组。然而，需要进一步进行更大样本量的研究和 HHV-8 蛋白表达的评估。
