Department of Oral Sciences "G. Messina", University of Palermo, 129 90127, Palermo, Italy.
Arch Immunol Ther Exp (Warsz). 2011 Dec;59(6):457-62. doi: 10.1007/s00005-011-0150-1. Epub 2011 Oct 1.
Oral lichen planus (OLP) is an autoimmune disease with an inflammatory pathogenesis. The angiogenetic phenomenon is a mechanism at the base of the pathogenesis of chronic inflammatory processes. The aim of this research is to evaluate the angiogenetic phenomenon, comparing an in vitro method with an in vivo one. Thirty OLP patients and 30 healthy subjects were enrolled in the study. Immunohistochemical analysis of the vascular-endothelial growth factor (VEGF) and vascular-endothelial adhesion molecules were carried out by the means of primary antibodies and anti-CD34, anti-VEGF, anti-CD106 antigen (VCAM-1) and anti-CD54 antigen (ICAM-1). Capillary density and others capillaroscopic parameters were tested in vivo using oral videocapillaroscopy. The results reveal the presence of a significant angiogenesis in OLP patients through the immunoexpression of VEGF, CD34, CD106 and CD54 (p < 0.001). Capillaroscopic analysis demonstrates significant value for the following parameters: density, tortuosity, loop diameter, afferent and efferent capillary loop diameter. The in vivo and in vitro investigation in OLP reveals a significant angiogenesis.
口腔扁平苔藓（OLP）是一种具有炎症发病机制的自身免疫性疾病。血管生成现象是慢性炎症过程发病机制的基础机制。本研究旨在通过体外方法与体内方法比较，评估血管生成现象。招募了 30 名 OLP 患者和 30 名健康受试者参加这项研究。通过使用主要抗体和抗-CD34、抗-VEGF、抗-CD106 抗原（VCAM-1）和抗-CD54 抗原（ICAM-1），进行血管内皮生长因子（VEGF）和血管内皮黏附分子的免疫组织化学分析。使用口腔视频毛细血管镜在体内测试毛细血管密度和其他毛细血管镜参数。结果表明，通过 VEGF、CD34、CD106 和 CD54 的免疫表达，OLP 患者存在明显的血管生成（p<0.001）。毛细血管镜分析表明以下参数具有显著价值：密度、迂曲度、环直径、输入和输出毛细血管环直径。OLP 的体内和体外研究表明存在明显的血管生成。
